PMID- 16808886
OWN - NLM
STAT- MEDLINE
DCOM- 20060912
LR  - 20151119
IS  - 0161-6412 (Print)
IS  - 0161-6412 (Linking)
VI  - 28
IP  - 5
DP  - 2006 Jul
TI  - Molecular markers of gliomas: a clinical approach.
PG  - 538-41
AB  - Over the last decade, the knowledge on the molecular genetic background of 
      gliomas has dramatically increased. This information provides the basis for the 
      molecular target therapies and molecular tests serve to complement the subjective 
      nature of histopathologic criteria and add useful data regarding response to 
      treatments and prognosis. In particular, the use of loss of heterozygosity (LOH) 
      and methylation specific polymerase chain reaction (PCR) (MSP) based testing of 
      gliomas is already in place and used clinically in several centers. This paper 
      provides a brief overview of these molecular genetic aberrations and discusses 
      the clinical utility, as well as the advantages and disadvantages of such 
      approach. Newly developed molecular techniques, such as LOH testing, fluorescence 
      in situ hybridization (FISH), DNA sequencing and MSP, are currently being 
      employed in assessment of gliomas in some laboratories. However, the clinical use 
      of some markers and the context in which the information obtained should be used 
      are still not entirely understood. Therefore, this paper will focus on validation 
      and implementation of molecular testing in gliomas, with emphasis on LOH on 
      chromosomes 1p, 19q, 17p and 10q and O(6)-methylguanine-DNA methyltransferase 
      (MGMT) methylation status.
FAU - Eoli, M
AU  - Eoli M
AD  - Neurological Institute C. Besta, Via Caloria 11, Milan, Italy.
FAU - Silvani, A
AU  - Silvani A
FAU - Pollo, B
AU  - Pollo B
FAU - Bianchessi, D
AU  - Bianchessi D
FAU - Menghi, F
AU  - Menghi F
FAU - Valletta, L
AU  - Valletta L
FAU - Broggi, G
AU  - Broggi G
FAU - Boiardi, A
AU  - Boiardi A
FAU - Bruzzone, M G
AU  - Bruzzone MG
FAU - Finocchiaro, G
AU  - Finocchiaro G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.1.1.63 (O(6)-Methylguanine-DNA Methyltransferase)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Brain Neoplasms/*genetics/metabolism
MH  - DNA Methylation
MH  - Diagnosis, Differential
MH  - Glioma/*genetics/metabolism
MH  - Humans
MH  - Loss of Heterozygosity
MH  - Molecular Biology/trends
MH  - O(6)-Methylguanine-DNA Methyltransferase/genetics/metabolism
MH  - Prognosis
RF  - 18
EDAT- 2006/07/01 09:00
MHDA- 2006/09/13 09:00
CRDT- 2006/07/01 09:00
PHST- 2006/07/01 09:00 [pubmed]
PHST- 2006/09/13 09:00 [medline]
PHST- 2006/07/01 09:00 [entrez]
AID - 10.1179/016164106X116827 [doi]
PST - ppublish
SO  - Neurol Res. 2006 Jul;28(5):538-41. doi: 10.1179/016164106X116827.