PMID- 16809218
OWN - NLM
STAT- MEDLINE
DCOM- 20060731
LR  - 20131121
IS  - 0190-2148 (Print)
IS  - 0190-2148 (Linking)
VI  - 32
IP  - 1-2
DP  - 2006 Jan-Feb
TI  - Doxycycline decreases monocyte chemoattractant protein-1 in human lung epithelial 
      cells.
PG  - 15-26
AB  - Certain antibiotics possess anti-inflammatory properties and could potentially be 
      used to treat inflammatory lung diseases associated with an influx of monocytes 
      such as panbronchiolitis, asthma, cystic fibrosis, and bronchitis. Doxycycline is 
      reported to possess anti-inflammatory effects. Monocyte chemoattractant protein-1 
      (MCP-1) is a major inflammatory cytokine and a powerful chemoattractant for 
      monocytes. The authors hypothesized that doxycycline exerts its anti-inflammatory 
      effects, in part, by reducing MCP-1 production. To test this hypothesis, A549 
      human lung epithelial cells were stimulated with cytomix in the presence or 
      absence of doxycycline. In stimulated cells doxycycline decreased MCP-1 
      production by 95% and in monocyte chemotaxis assays migration decreased by 55%. 
      However, doxycycline did decrease expression of MCP-1 mRNA and did not effect its 
      stability. These data demonstrate that doxycycline modulates MCP-1 production and 
      suggest that doxycycline may provide a new anti-inflammatory therapy for chronic 
      lung diseases.
FAU - Raza, Muhammad
AU  - Raza M
AD  - Research Service, Carl T. Hayden VA Medical Center, Phoenix, Arizona 85012, USA.
FAU - Ballering, Janelle G
AU  - Ballering JG
FAU - Hayden, John M
AU  - Hayden JM
FAU - Robbins, Richard A
AU  - Robbins RA
FAU - Hoyt, Jeffrey C
AU  - Hoyt JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Exp Lung Res
JT  - Experimental lung research
JID - 8004944
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - N12000U13O (Doxycycline)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Chemotaxis, Leukocyte/drug effects/immunology
MH  - Doxycycline/*pharmacology
MH  - Gene Expression/drug effects/immunology
MH  - Humans
MH  - Monocytes/cytology/immunology
MH  - Pneumonia/*drug therapy/immunology
MH  - RNA, Messenger/analysis
MH  - Respiratory Mucosa/*drug effects/immunology/metabolism
EDAT- 2006/07/01 09:00
MHDA- 2006/08/01 09:00
CRDT- 2006/07/01 09:00
PHST- 2006/07/01 09:00 [pubmed]
PHST- 2006/08/01 09:00 [medline]
PHST- 2006/07/01 09:00 [entrez]
AID - U888862PQ62L2H48 [pii]
AID - 10.1080/01902140600691399 [doi]
PST - ppublish
SO  - Exp Lung Res. 2006 Jan-Feb;32(1-2):15-26. doi: 10.1080/01902140600691399.