PMID- 16813572
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20190806
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 47
IP  - 4
DP  - 2006 Aug
TI  - The central coiled-coil domain and carboxyl-terminal WD-repeat domain of 
      Arabidopsis SPA1 are responsible for mediating repression of light signaling.
PG  - 564-76
AB  - Arabidopsis constitutive photomorphogenic 1 (COP1) is an E3 ubiquitin ligase that 
      directly binds and targets for degradation a number of 
      photomorphogenesis-promoting transcription factors, including HY5 and HFR1, to 
      desensitize light signaling. Arabidopsis suppressor of phyA-105 (SPA1), which 
      encodes a protein structurally related to COP1, also acts to repress 
      photomorphogenesis under various light conditions. Here we show that 
      overexpression of Arabidopsis SPA1 results in a hyperetiolation phenotype and 
      reduced accumulation of HY5 and HFR1. In addition, we show that both COP1 and 
      SPA1 are each dependent on the presence of the other for their repressive effect 
      on light signaling. Moreover, we demonstrate that both the central coiled-coil 
      and the C-terminal WD-repeat domains of SPA1 are necessary, and together these 
      two domains are sufficient for repressing photomorphogenesis. However, the 
      N-terminal kinase-like domain of SPA1 contributes to its full activity and 
      promotes destabilization of the SPA1 protein. Together, our results substantiate 
      the notion that COP1 and SPA1 act in concert to form a functional E3 ubiquitin 
      ligase complex and provide a molecular basis for understanding the 
      structure-function relationship of SPA1.
FAU - Yang, Jianping
AU  - Yang J
AD  - Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 
      14853, USA.
FAU - Wang, Haiyang
AU  - Wang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060630
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (SPA1 protein, Arabidopsis)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Arabidopsis/*metabolism/*radiation effects
MH  - Arabidopsis Proteins/*chemistry/genetics/*metabolism
MH  - Cell Cycle Proteins/*chemistry/genetics/*metabolism
MH  - Gene Expression Regulation, Plant
MH  - *Light
MH  - Protein Structure, Tertiary
MH  - Seedlings/genetics/growth & development
MH  - Signal Transduction/*radiation effects
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
EDAT- 2006/07/04 09:00
MHDA- 2007/02/28 09:00
CRDT- 2006/07/04 09:00
PHST- 2006/07/04 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2006/07/04 09:00 [entrez]
AID - TPJ2811 [pii]
AID - 10.1111/j.1365-313X.2006.02811.x [doi]
PST - ppublish
SO  - Plant J. 2006 Aug;47(4):564-76. doi: 10.1111/j.1365-313X.2006.02811.x. Epub 2006 
      Jun 30.