PMID- 16814989 OWN - NLM STAT- MEDLINE DCOM- 20061208 LR - 20060918 IS - 0923-2508 (Print) IS - 0923-2508 (Linking) VI - 157 IP - 8 DP - 2006 Oct TI - Exposure to sulfide causes populations shifts in sulfate-reducing consortia. PG - 784-91 AB - The shift in the community structure of a mixed culture of sulfate-reducing bacteria (SRB) at 0.5, 0.75, 1, and 1.5 kg m(-3) sulfide loadings was investigated in an anaerobic continuous bioreactor used for treatment of sulfate-containing wastewater by fluorescence in situ hybridization (FISH), using SRB species-specific and group-specific 16S rRNA-targeting probes. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes revealed that sulfide was toxic for Desulfonema, Desulfobulbus spp. and the Desulfobacteriaceae group, although it was not toxic for Desulfobacter, Desulfotomaculum, Desulfobacterium spp. or the Desulfovibrionaceae group. On the other hand, only a high concentration of sulfide of 1.5 kg m(-3) was found to be toxic for the Desulfococcus group in the bioreactor. When the sulfide in the feed was 1.00 kg m(-3) the sulfate-reducing capacity of the system decreased, and this decrease was more pronounced when the inlet sulfide was further increased to 1.5 kg m(-3). FAU - Icgen, Bulent AU - Icgen B AD - Bioprocess Engineering Research Unit, Department of Chemical Engineering, University of Cape Town, Rondebosch 7701, Cape Town, South Africa. bulent.icgen@tubitak.gov.tr FAU - Harrison, Sue AU - Harrison S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20060524 PL - France TA - Res Microbiol JT - Research in microbiology JID - 8907468 RN - 0 (RNA, Bacterial) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Sulfates) RN - 0 (Sulfides) SB - IM MH - Biodegradation, Environmental MH - Desulfitobacterium/drug effects/growth & development/*metabolism MH - In Situ Hybridization MH - RNA, Bacterial/*analysis MH - RNA, Ribosomal, 16S/*analysis MH - Sulfates/*metabolism MH - Sulfides/*pharmacology MH - Sulfur-Reducing Bacteria/drug effects/growth & development/*metabolism MH - Waste Disposal, Fluid EDAT- 2006/07/04 09:00 MHDA- 2006/12/12 09:00 CRDT- 2006/07/04 09:00 PHST- 2006/03/03 00:00 [received] PHST- 2006/04/11 00:00 [revised] PHST- 2006/04/26 00:00 [accepted] PHST- 2006/07/04 09:00 [pubmed] PHST- 2006/12/12 09:00 [medline] PHST- 2006/07/04 09:00 [entrez] AID - S0923-2508(06)00104-5 [pii] AID - 10.1016/j.resmic.2006.04.004 [doi] PST - ppublish SO - Res Microbiol. 2006 Oct;157(8):784-91. doi: 10.1016/j.resmic.2006.04.004. Epub 2006 May 24.