PMID- 16817218
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20181201
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 79
IP  - 3
DP  - 2006 Dec 1
TI  - Macrophage-mediated biodegradation of poly(DL-lactide-co-glycolide) in vitro.
PG  - 582-90
AB  - Biodegradation of poly-DL-lactide-co-glycolide (PLGA) both in vitro and in vivo 
      has been well documented. However, the roles that macrophages and their fused 
      multinucleated giant cells (MNGCs) play in this biodegradation are still unclear. 
      The current study aimed to investigate macrophage-mediated biodegradation of PLGA 
      thin films and of PLGA composites with hydroxyapatite (HA) and tricalcium 
      phosphate (TCP) ceramic powders in vitro using a murine macrophage cell line (RAW 
      264.7). The interactions were analyzed by using cell viability assays, scanning 
      electron microscopy, and focused ion beam microscopy. The results showed that RAW 
      264.7 cells effectively attached and proliferated on the PLGA films and PLGA-HA, 
      PLGA-TCP composites. The RAW 264.7 cells were observed to aggregate and fuse to 
      form MNGCs. The cell processes on the membrane, or pseudopodia, penetrated into 
      the PLGA films and evidently eroded the surface. We conclude that macrophages and 
      fused MNGCs actively respond to PLGA films as substratum and degrade the surface 
      of this polymer.
FAU - Xia, Zhidao
AU  - Xia Z
AD  - Nuffield Department of Orthopaedic Surgery, The Botnar Research Centre, Institute 
      of Musculoskeletal Sciences, University of Oxford, Oxford, United Kingdom.
FAU - Huang, Yizhong
AU  - Huang Y
FAU - Adamopoulos, Iannis E
AU  - Adamopoulos IE
FAU - Walpole, Andrew
AU  - Walpole A
FAU - Triffitt, James T
AU  - Triffitt JT
FAU - Cui, Zhanfeng
AU  - Cui Z
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Calcium Phosphates)
RN  - 0 (Polymers)
RN  - 0 (Solvents)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - K4C08XP666 (tricalcium phosphate)
SB  - IM
MH  - Animals
MH  - Calcium Phosphates/pharmacology
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Lactic Acid/*metabolism/toxicity
MH  - Macrophages/cytology/drug effects/*metabolism
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Polyglycolic Acid/*metabolism/toxicity
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*metabolism/toxicity
MH  - Solvents
EDAT- 2006/07/04 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/07/04 09:00
PHST- 2006/07/04 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/07/04 09:00 [entrez]
AID - 10.1002/jbm.a.30853 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2006 Dec 1;79(3):582-90. doi: 10.1002/jbm.a.30853.