PMID- 16817771
OWN - NLM
STAT- MEDLINE
DCOM- 20060928
LR  - 20061115
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 19
IP  - 2
DP  - 2006 Summer
TI  - Neutralizing antibody formation in swine infected with seven strains of porcine 
      reproductive and respiratory syndrome virus as measured by indirect ELISA with 
      peptides containing the GP5 neutralization epitope.
PG  - 285-93
AB  - An indirect ELISA with peptides containing the GP5 neutralization epitope was 
      used to measure the time courses of formation of neutralizing antibodies in sera 
      of groups of 10 pigs infected with seven different strains of porcine 
      reproductive and respiratory syndrome virus (PRRSV) generated in an earlier study 
      (Johnson et al., Vet.Immunol. Immunopath. 102:233-247. 2004). The neutralizing 
      antibody responses varied greatly between individual pigs infected with each 
      PRRSV strain. Some pigs generated high titers of neutralizing antibodies between 
      7 and 28 days post infection (p.i), whereas other pigs had not generated a 
      significant response by 42 days p.i. The heterogeneity in antibody formation in 
      individual pigs also pertained to anti-N-protein antibody formation as measured 
      by both HerdCheck and peptide ELISAs and there was no correlation between 
      anti-N-protein and neutralizing antibody formation. Also, viremia was 
      disconnected from neutralizing antibody formation. Viremia peaked 7-15 days p.i. 
      and then precipitously declined to undetectable levels in most infected pigs by 
      21-28 day p.i. whether or not they had generated neutralizing antibodies. 
      Furthermore, contrary to an earlier conclusion from this study the formation of 
      neither neutralizing nor anti-N-protein antibodies was related to viral load as 
      measured by infectious virus levels in serum.
FAU - Plagemann, Peter G W
AU  - Plagemann PG
AD  - Department of Microbiology, Medical School, University of Minnesota, Minneapolis, 
      Minnesota 55455-0312, USA. plage001@umn.edu
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Peptides)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/*biosynthesis/blood/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/chemistry/*immunology
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Peptides/chemistry/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/*immunology/pathogenicity
MH  - Swine
MH  - Viral Envelope Proteins/chemistry/*immunology
MH  - Viral Load
EDAT- 2006/07/05 09:00
MHDA- 2006/09/29 09:00
CRDT- 2006/07/05 09:00
PHST- 2006/07/05 09:00 [pubmed]
PHST- 2006/09/29 09:00 [medline]
PHST- 2006/07/05 09:00 [entrez]
AID - 10.1089/vim.2006.19.285 [doi]
PST - ppublish
SO  - Viral Immunol. 2006 Summer;19(2):285-93. doi: 10.1089/vim.2006.19.285.