PMID- 16817772
OWN - NLM
STAT- MEDLINE
DCOM- 20060928
LR  - 20061115
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 19
IP  - 2
DP  - 2006 Summer
TI  - Neutralizing antibody responses of pigs infected with natural GP5 N-glycan 
      mutants of porcine reproductive and respiratory syndrome virus.
PG  - 294-304
AB  - In order to assess the effect of the N-glycans associated with the GP5 
      neutralization epitope of porcine reproductive and respiratory syndrome virus 
      (PRRSV) on the neutralizing antibody (Ab) response of swine, groups of young pigs 
      were infected with PRRSV strains differing in N-glycosylation pattern. The 
      humoral immune response to strain VR-2332, harboring four potential N-glycan 
      sites, was compared to that of two natural field isolates carrying mutations 
      either abolishing the N-glycosylation site at position 44 (N44) or the two 
      N-glycosylation sites in the hypervariable region upstream of the neutralization 
      epitope (HV-1). The pigs were bled at intervals and their sera were assayed for 
      neutralizing Abs by indirect and competition ELISAs using peptides containing the 
      GP5 neutralization epitope, and selectively for infectivity neutralization of a 
      number of PRRSV strains. In addition, viremia was monitored by quantitative 
      RT-PCR, and anti-N-protein Ab formation was measured by HerdChek ELISA. The 
      neutralizing Ab responses as measured by peptide ELISA varied greatly between 
      individual pigs infected with each PRRSV strain. Some pigs generated high titers 
      of peptide binding Abs between 7 and 28 days post infection (p.i.), whereas other 
      pigs had not generated a response by 90 days p.i. However, the HV-1-infected pigs 
      generated Abs to the neutralization epitope more rapidly and to a 5-10 times 
      higher level than VR-2332-infected pigs, and the Abs neutralized the homologous 
      HV-1 virus 10-20 times more efficiently than PRRSV strains VR-2332, N44, MN184, 
      or SDSU73. In contrast, most N44-infected pigs generated neutralizing Abs only 
      after 42 days p.i. and only to low levels. The results suggest that the deletions 
      of the N-glycans or other amino acid substitutions in the GP5 ectodomains of the 
      mutants affect the immunogenicity of the neutralization epitope and the 
      specificity of the Abs raised to it but not the sensitivity of the virions to Ab 
      neutralization.
FAU - Faaberg, Kay S
AU  - Faaberg KS
AD  - Department of Veterinary and Biological Sciences, College of Veterinary Medicine, 
      University of Minnesota, Saint Paul, Minnesota 55455, USA.
FAU - Hocker, Jason D
AU  - Hocker JD
FAU - Erdman, Matthew M
AU  - Erdman MM
FAU - Harris, D L Hank
AU  - Harris DL
FAU - Nelson, Eric A
AU  - Nelson EA
FAU - Torremorell, Montserrat
AU  - Torremorell M
FAU - Plagemann, Peter G W
AU  - Plagemann PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Peptides)
RN  - 0 (Polysaccharides)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/*blood/immunology
MH  - Cell Line
MH  - Epitopes
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Neutralization Tests
MH  - Peptides/chemical synthesis/chemistry/immunology
MH  - Polysaccharides/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*immunology/pathogenicity
MH  - Swine
MH  - Viral Envelope Proteins/genetics/*immunology
EDAT- 2006/07/05 09:00
MHDA- 2006/09/29 09:00
CRDT- 2006/07/05 09:00
PHST- 2006/07/05 09:00 [pubmed]
PHST- 2006/09/29 09:00 [medline]
PHST- 2006/07/05 09:00 [entrez]
AID - 10.1089/vim.2006.19.294 [doi]
PST - ppublish
SO  - Viral Immunol. 2006 Summer;19(2):294-304. doi: 10.1089/vim.2006.19.294.