PMID- 16817774
OWN - NLM
STAT- MEDLINE
DCOM- 20060928
LR  - 20181113
IS  - 0882-8245 (Print)
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 19
IP  - 2
DP  - 2006 Summer
TI  - Differential expression of ovine innate immune genes by preterm and neonatal lung 
      epithelia infected with respiratory syncytial virus.
PG  - 316-23
AB  - Preterm infants have increased susceptibility to severe manifestations of 
      respiratory syncytial virus (RSV) infection. The cause(s) for this age-dependent 
      vulnerability is/are not well-defined, but alterations in innate immune products 
      have been implicated. In sheep, RSV disease severity has similar age-dependent 
      characteristics and sheep have several related innate molecules for study during 
      pulmonary infection including surfactant protein A (SP-A), surfactant protein D 
      (SP-D), sheep beta defensin 1 (SBD1), monocyte chemotactic protein 1 (MCP1), and 
      Toll-like receptor 4 (TLR4). However, the in vivo cellular gene expression as a 
      response to RSV infection is poorly understood. In this study, the effect of RSV 
      infection on expression of these innate immune genes was determined for bovine 
      RSV-infected (bRSV+ fluorescence) epithelial cells, adjacent cells lacking bRSV 
      antigen (adjoining cells lacking fluorescence), and control cells from 
      non-infected lung using laser capture microdissection (LCM) and real-time RT-PCR. 
      Control lambs had increased expression of innate immune molecules in full term 
      (term) compared to preterm epithelia with statistical significance in SBD1, SP-D, 
      and TLR4 mRNA. Infected cells (bRSV+ fluorescent cells) had consistently higher 
      mRNA levels of SP-A (preterm and term), MCP1 (preterm and term), and SP-D 
      (preterm). Interestingly, bRSV- cells of infected term lambs had significantly 
      reduced SP-D mRNA expression compared to bRSV+ and control epithelia, suggesting 
      that RSV infected cells may regulate the adjacent epithelial SP-D expression. 
      This study defines specific innate immune components (e.g., SBD1, SP-D, and TLR4) 
      that have differential age-dependent expression in the airway epithelia. 
      Furthermore, cellular bRSV infection enhanced certain innate immune components 
      while suppressing adjacent cellular SP-D expression in term animals. These in 
      vivo gene expression results provide a framework for future studies on 
      age-dependent susceptibility to RSV and RSV pathogenesis.
FAU - Kawashima, Kenji
AU  - Kawashima K
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State 
      University Ames, Iowa 50011-1250, USA.
FAU - Meyerholz, David K
AU  - Meyerholz DK
FAU - Gallup, Jack M
AU  - Gallup JM
FAU - Grubor, Branka
AU  - Grubor B
FAU - Lazic, Tatjana
AU  - Lazic T
FAU - Lehmkuhl, Howard D
AU  - Lehmkuhl HD
FAU - Ackermann, Mark R
AU  - Ackermann MR
LA  - eng
GR  - R01 AI062787-04/AI/NIAID NIH HHS/United States
GR  - R01 AI062787/AI/NIAID NIH HHS/United States
GR  - 5K08AI055499-03/AI/NIAID NIH HHS/United States
GR  - K08 AI055499/AI/NIAID NIH HHS/United States
GR  - 05R01AI062787-02/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - *Epithelial Cells/immunology/metabolism/virology
MH  - *Gene Expression Regulation, Developmental
MH  - Immunity, Innate/*genetics
MH  - Lasers
MH  - Lung/cytology/*virology
MH  - Microdissection/methods
MH  - Premature Birth/veterinary
MH  - RNA, Messenger/genetics/metabolism
MH  - Respiratory Syncytial Virus Infections/immunology/veterinary/virology
MH  - Respiratory Syncytial Viruses/*pathogenicity
MH  - Sheep
MH  - Sheep Diseases/*immunology/virology
PMC - PMC2791066
MID - NIHMS160733
EDAT- 2006/07/05 09:00
MHDA- 2006/09/29 09:00
PMCR- 2009/12/09
CRDT- 2006/07/05 09:00
PHST- 2006/07/05 09:00 [pubmed]
PHST- 2006/09/29 09:00 [medline]
PHST- 2006/07/05 09:00 [entrez]
PHST- 2009/12/09 00:00 [pmc-release]
AID - 10.1089/vim.2006.19.316 [doi]
PST - ppublish
SO  - Viral Immunol. 2006 Summer;19(2):316-23. doi: 10.1089/vim.2006.19.316.