PMID- 16817859
OWN - NLM
STAT- MEDLINE
DCOM- 20060804
LR  - 20061115
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 23
IP  - 10
DP  - 2006 May
TI  - Expression of pheromone receptor gene families during olfactory development in 
      the mouse: expression of a V1 receptor in the main olfactory epithelium.
PG  - 2563-72
AB  - In the mouse, two large gene families, V1R and V2R, encoding putative pheromone 
      receptors have been described. Studies have suggested a homotypic recognition 
      role for V1Rs and V2Rs during development in the targeting of vomeronasal axons 
      to specific sets of glomeruli in the accessory olfactory bulb (AOB). Analysis of 
      the onset of expression of the V1R and V2R gene families in developing 
      vomeronasal neurons using polymerase chain reaction and in situ hybridization now 
      suggests that a role for these receptors in the organization of axon projections 
      is only likely at the final stages of targeting within the AOB. Surprisingly, our 
      studies reveal expression of a V1Rd receptor in scattered cells within the main 
      olfactory epithelium, suggesting that limited pheromone detection may also take 
      place in this structure. The pheromone sensory neurons of the vomeronasal system 
      and the neuroendocrine gonadotrophin-releasing hormone (GnRH) neurons that 
      regulate fertility both arise from progenitor cells of the nasal placode. The 
      development of these two cell types is intimately linked, and the GnRH neuron 
      population migrates into the forebrain during embryogenesis in close association 
      with a subset of vomeronasal sensory axons; how GnRH neurons recognize this axon 
      subset is unknown. We report selective expression of a V1Ra gene in the clonal 
      NLT GnRH cell line, raising the possibility of a similar role for V1Rs or V2Rs in 
      the directed migration of GnRH neurons. However, no expression of this gene or of 
      other V1Rs and V2Rs is detectable at the cellular level in migrating GnRH neurons 
      in the mouse.
FAU - Karunadasa, Delicia K
AU  - Karunadasa DK
AD  - Laboratory of Neuronal Development & Survival, The Babraham Institute, Babraham 
      Research Campus, Cambridge CB2 4AT, UK.
FAU - Chapman, Christopher
AU  - Chapman C
FAU - Bicknell, R John
AU  - Bicknell RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Pheromone)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Movement/physiology
MH  - Gene Expression
MH  - *Gene Expression Regulation, Developmental
MH  - Gonadotropin-Releasing Hormone/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Mice
MH  - Neurons/*cytology/metabolism
MH  - Olfactory Bulb/*embryology
MH  - Olfactory Mucosa/cytology/*physiology
MH  - RNA, Messenger/analysis
MH  - Receptors, Pheromone/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vomeronasal Organ/cytology/*embryology/innervation
EDAT- 2006/07/05 09:00
MHDA- 2006/08/05 09:00
CRDT- 2006/07/05 09:00
PHST- 2006/07/05 09:00 [pubmed]
PHST- 2006/08/05 09:00 [medline]
PHST- 2006/07/05 09:00 [entrez]
AID - EJN4795 [pii]
AID - 10.1111/j.1460-9568.2006.04795.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2006 May;23(10):2563-72. doi: 10.1111/j.1460-9568.2006.04795.x.