PMID- 16819333
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20220408
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 30
IP  - 7
DP  - 2006 Jul
TI  - Ultrasonic decalcification offers new perspectives for rapid FISH, DNA, and 
      RT-PCR analysis in bone marrow trephines.
PG  - 892-6
AB  - The requisite analyses on bone marrow biopsies are increasing: Molecular analyses 
      such as fluorescence in situ hybridization (FISH), polymerase chain reaction 
      (PCR), and reverse transcriptase (RT)-PCR are demanded in addition to morphology 
      and immunohistochemistry to improve diagnostic accuracy. Moreover, analysis of 
      certain molecular prognostic or predictive biomarkers is increasingly mandatory 
      in the assessment of hematologic diseases. In some circumstances, only formalin 
      fixed, bone-containing tissue is available for molecular analysis. Because 
      various fixation and decalcification procedures can impair DNA and RNA quality, 
      there is an urgent need for standardized decalcification protocols which allow 
      FISH and PCR analysis. In this study we developed a routinely applicable 
      decalcification protocol to optimize the molecular analysis method although 
      preserving morphology and immunohistochemical results. Therefore, we compared 2 
      different approaches including ultrasonic decalcification versus nonultrasonic 
      procedures and ethylenediaminetetraacetate-based reagents versus acid-based ones. 
      In our hands, the combined use of ultrasound and 
      ethylenediaminetetraacetate-based reagents permits successful interphase FISH, 
      PCR, and RT-PCR analysis whereas concomitantly preserving morphology and 
      antigeneicity.
FAU - Reineke, Tanja
AU  - Reineke T
AD  - Institute of Surgical Pathology, University Hospital Zurich, Schmelsburgstrasse 
      12, CH-8091 Zurich, Switzerland.
FAU - Jenni, Bettina
AU  - Jenni B
FAU - Abdou, Marie-Therese
AU  - Abdou MT
FAU - Frigerio, Simona
AU  - Frigerio S
FAU - Zubler, Petra
AU  - Zubler P
FAU - Moch, Holger
AU  - Moch H
FAU - Tinguely, Marianne
AU  - Tinguely M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Fixatives)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 411VRN1TV4 (Nitric Acid)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Biopsy
MH  - Bone Marrow Examination/methods
MH  - Calcium/*chemistry
MH  - DNA/*analysis
MH  - *Decalcification Technique
MH  - Edetic Acid/chemistry
MH  - Fixatives
MH  - Formaldehyde
MH  - *In Situ Hybridization, Fluorescence
MH  - Nitric Acid/chemistry
MH  - Paraffin Embedding
MH  - RNA/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Specimen Handling/*methods
MH  - Tissue Fixation
MH  - Ultrasonics
EDAT- 2006/07/05 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/07/05 09:00
PHST- 2006/07/05 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/07/05 09:00 [entrez]
AID - 00000478-200607000-00015 [pii]
AID - 10.1097/01.pas.0000213282.20166.13 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2006 Jul;30(7):892-6. doi: 10.1097/01.pas.0000213282.20166.13.