PMID- 16820475
OWN - NLM
STAT- MEDLINE
DCOM- 20060905
LR  - 20240315
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 7
DP  - 2006 Jul
TI  - Optimization of a sampling system for recovery and detection of airborne porcine 
      reproductive and respiratory syndrome virus and swine influenza virus.
PG  - 4811-8
AB  - The objective of this research was to optimize sampling parameters for increased 
      recovery and detection of airborne porcine reproductive and respiratory syndrome 
      virus (PRRSV) and swine influenza virus (SIV). Collection media containing 
      antifoams, activated carbons, protectants, and ethylene glycol were evaluated for 
      direct effects on factors impacting the detection of PRRSV and SIV, including 
      virus infectivity, viability of continuous cell lines used for the isolation of 
      these viruses, and performance of reverse transcriptase PCR assays. The results 
      showed that specific compounds influenced the likelihood of detecting PRRSV and 
      SIV in collection medium. A subsequent study evaluated the effects of collection 
      medium, impinger model, and sampling time on the recovery of aerosolized PRRSV 
      using a method for making direct comparisons of up to six treatments 
      simultaneously. The results demonstrated that various components in air-sampling 
      systems, including collection medium, impinger model, and sampling time, 
      independently influenced the recovery and detection of PRRSV and/or SIV. 
      Interestingly, it was demonstrated that a 20% solution of ethylene glycol 
      collected the greatest quantity of aerosolized PRRSV, which suggests the 
      possibility of sampling at temperatures below freezing. Based on the results of 
      these experiments, it is recommended that air-sampling systems be optimized for 
      the target pathogen(s) and that recovery/detection results should be interpreted 
      in the context of the actual performance of the system.
FAU - Hermann, J R
AU  - Hermann JR
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA.
FAU - Hoff, S J
AU  - Hoff SJ
FAU - Yoon, K J
AU  - Yoon KJ
FAU - Burkhardt, A C
AU  - Burkhardt AC
FAU - Evans, R B
AU  - Evans RB
FAU - Zimmerman, J J
AU  - Zimmerman JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Antifoaming Agents)
RN  - 0 (Culture Media)
RN  - 0 (Ethylene Glycols)
SB  - IM
MH  - *Air Microbiology
MH  - Animals
MH  - Antifoaming Agents/pharmacology
MH  - Cell Line
MH  - Culture Media
MH  - Ethylene Glycols/pharmacology
MH  - Influenza A virus/genetics/*isolation & purification/pathogenicity
MH  - Orthomyxoviridae Infections/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification/pathogenicity
MH  - Specimen Handling/*methods
MH  - Swine
MH  - Swine Diseases/virology
MH  - Virology/methods
PMC - PMC1489351
EDAT- 2006/07/06 09:00
MHDA- 2006/09/06 09:00
PMCR- 2006/11/01
CRDT- 2006/07/06 09:00
PHST- 2006/07/06 09:00 [pubmed]
PHST- 2006/09/06 09:00 [medline]
PHST- 2006/07/06 09:00 [entrez]
PHST- 2006/11/01 00:00 [pmc-release]
AID - 72/7/4811 [pii]
AID - 0472-06 [pii]
AID - 10.1128/AEM.00472-06 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Jul;72(7):4811-8. doi: 10.1128/AEM.00472-06.