PMID- 16820953
OWN - NLM
STAT- MEDLINE
DCOM- 20070118
LR  - 20131121
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 73
IP  - 2
DP  - 2006 Nov
TI  - High-yield 5-keto-D-gluconic acid formation is mediated by soluble and 
      membrane-bound gluconate-5-dehydrogenases of Gluconobacter oxydans.
PG  - 443-51
AB  - Gluconobacter oxydans DSM 2343 is known to catalyze the oxidation of glucose to 
      gluconic acid, and subsequently, to 2-keto-D-gluconic acid (2-KGA) and 
      5-keto-D-gluconic acid (5-KGA), by membrane-bound and soluble dehydrogenases. In 
      G. oxydans MF1, in which the membrane-bound gluconate-2-dehydrogenase complex was 
      inactivated, formation of the undesired 2-KGA was absent. This mutant strain 
      uniquely accumulates high amounts of 5-KGA in the culture medium. To increase the 
      production rate of 5-KGA, which can be converted to industrially important 
      L-(+)-tartaric acid, we equipped G. oxydans MF1 with plasmids allowing the 
      overproduction of the soluble and the membrane-bound 5-KGA-forming enzyme. 
      Whereas the overproduction of the soluble gluconate:NADP 5-oxidoreductase 
      resulted in the accumulation of up to 200 mM 5-KGA, the detected 5-KGA 
      accumulation was even higher when the gene coding for the membrane-bound 
      gluconate-5-dehydrogenase was overexpressed (240 to 295 mM 5-KGA). These results 
      provide a basis for designing a biotransformation process for the conversion of 
      glucose to 5-KGA using the membrane-bound as well as the soluble enzyme system.
FAU - Merfort, Marcel
AU  - Merfort M
AD  - Institut fur Biotechnologie 1, Forschungszentrum Julich GmbH, 52425 Julich, 
      Germany.
FAU - Herrmann, Ute
AU  - Herrmann U
FAU - Bringer-Meyer, Stephanie
AU  - Bringer-Meyer S
FAU - Sahm, Hermann
AU  - Sahm H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060705
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Acetates)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Gluconates)
RN  - 0 (Tartrates)
RN  - 7440-44-0 (Carbon)
RN  - 815-89-4 (5-ketogluconic acid)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.1.1.69 (gluconate 5-dehydrogenase)
RN  - IY9XDZ35W2 (Glucose)
RN  - R4R8J0Q44B (gluconic acid)
RN  - S88TT14065 (Oxygen)
RN  - W4888I119H (tartaric acid)
SB  - IM
MH  - Acetates/chemistry
MH  - Bacterial Proteins/chemistry/*physiology
MH  - Biotechnology/*methods
MH  - Carbon/chemistry
MH  - Fermentation
MH  - Gene Expression Regulation, Bacterial
MH  - Genes, Bacterial
MH  - Gluconates/*chemistry/metabolism
MH  - Gluconobacter oxydans/*enzymology
MH  - Glucose/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Oxidoreductases/chemistry/*physiology
MH  - Oxygen/chemistry/metabolism
MH  - Plasmids/metabolism
MH  - Tartrates/chemistry
MH  - Time Factors
EDAT- 2006/07/06 09:00
MHDA- 2007/01/19 09:00
CRDT- 2006/07/06 09:00
PHST- 2006/02/06 00:00 [received]
PHST- 2006/04/19 00:00 [accepted]
PHST- 2006/04/10 00:00 [revised]
PHST- 2006/07/06 09:00 [pubmed]
PHST- 2007/01/19 09:00 [medline]
PHST- 2006/07/06 09:00 [entrez]
AID - 10.1007/s00253-006-0467-6 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2006 Nov;73(2):443-51. doi: 10.1007/s00253-006-0467-6. 
      Epub 2006 Jul 5.