PMID- 16822516
OWN - NLM
STAT- MEDLINE
DCOM- 20090417
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1130
IP  - 1
DP  - 2006 Oct 13
TI  - Determination of antimicrobials in sludge from infiltration basins at two 
      artificial recharge plants by pressurized liquid extraction-liquid 
      chromatography-tandem mass spectrometry.
PG  - 72-82
AB  - This work describes the optimization of a multi-residue analytical approach for 
      the simultaneous determination of 11 antimicrobials (9 sulphonamides and 2 
      penicillins) in sludge from infiltration basins. The method is based on 
      pressurized liquid extraction (PLE) followed by solid-phase extraction (SPE) for 
      pre-concentration and purification, and analysis by liquid chromatography-tandem 
      mass spectrometry using electrospray in the positive ionization mode 
      (LC-(ESI+)-MS/MS). Limits of detections (LODs) between 1 pg/g and 0.2 ng/g and 
      limits of quantifications (LOQs) between 5 pg/g and 0.6 ng/g were achieved. Good 
      recovery values (57.6-104%) were obtained for sulfamethazine, sulfapyridine, 
      sulfadiazine and sulfamethoxypyridazine, while medium recovery values (14-47%) 
      were afforded for sulfadimethoxine, sulfathiazole and sulfamethoxazole. However, 
      only a poor recovery (<1%) could be possible for both penicillins and two 
      sulphonamides, namely nafcillin, dicloxacillin, sulfisoxazole and sulfamethizole. 
      These low recoveries were attributed to the presence of ionic suppression effects 
      (even after thorough extraction and purification) rather than to an inefficient 
      extraction. The method developed was applied to the analysis of sludge samples 
      from the infiltration basins of two artificial recharge plants located in Sweden 
      and Denmark. All target compounds were found to be present in at least one 
      sample. Sulfadimethoxine, nafcillin and dicloxacillin were detected in all the 
      samples analysed.
FAU - Diaz-Cruz, M Silvia
AU  - Diaz-Cruz MS
AD  - Department of Environmental Chemistry, Instituto de Investigaciones Quimicas y 
      Ambientales, Consejo Superior de Investigaciones Cientificas, c/Jordi Girona 
      18-26, 08034 Barcelona, Spain. sdcqam@cid.csic.es
FAU - Lopez de Alda, Maria Jose
AU  - Lopez de Alda MJ
FAU - Barcelo, Damia
AU  - Barcelo D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20060705
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Sewage)
SB  - IM
MH  - Anti-Infective Agents/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Denmark
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Sewage/*analysis
MH  - Solid Phase Extraction/*methods
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Sweden
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2006/07/11 09:00
MHDA- 2009/04/18 09:00
CRDT- 2006/07/11 09:00
PHST- 2006/03/14 00:00 [received]
PHST- 2006/05/18 00:00 [revised]
PHST- 2006/05/19 00:00 [accepted]
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2009/04/18 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
AID - S0021-9673(06)01075-2 [pii]
AID - 10.1016/j.chroma.2006.05.076 [doi]
PST - ppublish
SO  - J Chromatogr A. 2006 Oct 13;1130(1):72-82. doi: 10.1016/j.chroma.2006.05.076. 
      Epub 2006 Jul 5.