PMID- 16822961
OWN - NLM
STAT- MEDLINE
DCOM- 20061212
LR  - 20191210
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 291
IP  - 6
DP  - 2006 Dec
TI  - An oxidized metabolite of linoleic acid increases intracellular calcium in rat 
      adrenal glomerulosa cells.
PG  - E1160-7
AB  - EKODE, an epoxy-keto derivative of linoleic acid, was previously shown to 
      stimulate aldosterone secretion in rat adrenal glomerulosa cells. In the present 
      study, we investigated the effect of exogenous EKODE on cytosolic [Ca(2+)] 
      increase and aimed to elucidate the mechanism involved in this process. Through 
      the use of the fluorescent Ca(2+)-sensitive dye Fluo-4, EKODE was shown to 
      rapidly increase intracellular [Ca(2+)] ([Ca(2+)](i)) along a bell-shaped 
      dose-response relationship with a maximum peak at 5 microM. Experiments performed 
      in the presence or absence of Ca(2+) revealed that this increase in [Ca(2+)](i) 
      originated exclusively from intracellular pools. EKODE-induced [Ca(2+)](i) 
      increase was blunted by prior application of angiotensin II, Xestospongin C, and 
      cyclopiazonic acid, indicating that inositol trisphosphate (InsP(3))-sensitive 
      Ca(2+) stores can be mobilized by EKODE despite the absence of InsP(3) 
      production. Accordingly, EKODE response was not sensitive to the phospholipase C 
      inhibitor U-73122. EKODE mobilized a Ca(2+) store included in the thapsigargin 
      (TG)-sensitive stores, although the interaction between EKODE and TG appears 
      complex, since EKODE added at the plateau response of TG induced a rapid drop in 
      [Ca(2+)](i). 9-oxo-octadecadienoic acid, another oxidized derivative of linoleic 
      acid, also increases [Ca(2+)](i), with a dose-response curve similar to EKODE. 
      However, arachidonic and linoleic acids at 10 microM failed to increase 
      [Ca(2+)](i) but did reduce the amplitude of the response to EKODE. It is 
      concluded that EKODE mobilizes Ca(2+) from an InsP(3)-sensitive store and that 
      this [Ca(2+)](i) increase is responsible for aldosterone secretion by glomerulosa 
      cells. Similar bell-shaped dose-response curves for aldosterone and [Ca(2+)](i) 
      increases reinforce this hypothesis.
FAU - Payet, Marcel D
AU  - Payet MD
AD  - Department of Physiology, University of Sherbrooke, QC, Canada J1H 5N4. 
      marcel.payet@usherbrooke.ca
FAU - Goodfriend, Theodore L
AU  - Goodfriend TL
FAU - Bilodeau, Lyne
AU  - Bilodeau L
FAU - Mackendale, Cherilu
AU  - Mackendale C
FAU - Chouinard, Lucie
AU  - Chouinard L
FAU - Gallo-Payet, Nicole
AU  - Gallo-Payet N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060705
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (12,13-epoxy-9- keto-10-octadecenoic acid)
RN  - 0 (Fatty Acids)
RN  - 0 (Oleic Acids)
RN  - 0 (Steroids)
RN  - 11128-99-7 (Angiotensin II)
RN  - 132-06-9 (Inosine Triphosphate)
RN  - 4964P6T9RB (Aldosterone)
RN  - 9KJL21T0QJ (Linoleic Acid)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aldosterone/metabolism
MH  - Angiotensin II/pharmacology
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/metabolism
MH  - Cyclic AMP/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Fatty Acids/metabolism
MH  - In Vitro Techniques
MH  - Inosine Triphosphate/biosynthesis
MH  - Linoleic Acid/metabolism/*pharmacology
MH  - Oleic Acids/metabolism/*pharmacology
MH  - Oxidation-Reduction
MH  - Rats
MH  - Signal Transduction/drug effects
MH  - Steroids/biosynthesis
MH  - Zona Glomerulosa/cytology/drug effects/*metabolism
EDAT- 2006/07/11 09:00
MHDA- 2006/12/13 09:00
CRDT- 2006/07/11 09:00
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2006/12/13 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
AID - 00108.2006 [pii]
AID - 10.1152/ajpendo.00108.2006 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2006 Dec;291(6):E1160-7. doi: 
      10.1152/ajpendo.00108.2006. Epub 2006 Jul 5.