PMID- 16823071
OWN - NLM
STAT- MEDLINE
DCOM- 20070130
LR  - 20191110
IS  - 1661-7827 (Print)
IS  - 1660-4601 (Electronic)
IS  - 1660-4601 (Linking)
VI  - 3
IP  - 1
DP  - 2006 Mar
TI  - Comparative study of Domoic Acid and Okadaic Acid induced-chromosomal 
      abnormalities in the Caco-2 cell line.
PG  - 4-10
AB  - Okadaic Acid (OA) the major diarrheic shellfish poisoning (DSP) toxin is known as 
      a tumor promoter and seems likely implicated in the genesis of digestive cancer. 
      Little is known regarding genotoxicity and carcinogenicity of Domoic Acid (DA), 
      the major Amnesic Shellfish Poisoning (ASP) toxin. Both OA and DA occur in 
      seafood and are of human health concerns. Micronuclei (MN) arise from 
      abnormalities in nuclear division during mitosis due to a failure of the mitotic 
      spindle or by complex chromosomal configurations that pose problems during 
      anaphase. In order to evaluate the ability of okadaic acid (OA) and domoic acid 
      (DA) to induce DNA damage we performed the micronucleus assay using the Caco-2 
      cell line. To discriminate between a clastogenic or aneugenic effect of OA and 
      DA, the micronucleus assay was conducted by cytokinesis-block micronucleus assay 
      using cytochalasin B with Giemsa staining and/or acridine orange staining, in 
      parallel to fluorescence in situ hybridization (FISH) using a concentrated human 
      pan-centromeric chromosome paint probe. Our results showed that OA and DA 
      significantly increased the frequency of MN in Caco-2 cells. The MN caused by OA 
      are found in mononucleated cells and binucleated cells, whereas those caused by 
      DA are mainly in binucleated cells. The results of FISH analysis showed that OA 
      induced centromere-positive micronuclei and DA increased the percentage of MN 
      without a centromeric signal. In conclusion, both OA and DA bear mutagenic 
      potential as revealed in Caco-2 cells by induction of MN formation. Moreover, OA 
      induced whole chromosome loss suggesting a specific aneugenic potential, whereas 
      DA seems simply clastogenic. At present, one cannot rule out possible DNA damage 
      of intestinal cells if concentrations studied are reached in vivo, since this may 
      happen with concentrations of toxins just below regulatory limits in case of 
      frequent consumption of contaminated shell fishes.
FAU - Carvalho, Pinto-Silva
AU  - Carvalho PS
AD  - Department of Environmental Engineering, Toxicological Laboratory of the Federal 
      University of Santa Catarina, Brazil Campus Universitario, Trindade 88040-900, 
      Brazil.
FAU - Catian, R
AU  - Catian R
FAU - Moukha, Serge
AU  - Moukha S
FAU - Matias, William G
AU  - Matias WG
FAU - Creppy, Edmond E
AU  - Creppy EE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Int J Environ Res Public Health
JT  - International journal of environmental research and public health
JID - 101238455
RN  - 0 (Mutagens)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - M02525818H (domoic acid)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Caco-2 Cells
MH  - *Chromosome Aberrations
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kainic Acid/*analogs & derivatives/toxicity
MH  - Micronucleus Tests
MH  - Mutagens/*toxicity
MH  - Okadaic Acid/*toxicity
PMC - PMC3785674
EDAT- 2006/07/11 09:00
MHDA- 2007/01/31 09:00
PMCR- 2006/03/01
CRDT- 2006/07/11 09:00
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2007/01/31 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
PHST- 2006/03/01 00:00 [pmc-release]
AID - ijerph-03-00004 [pii]
AID - 10.3390/ijerph2006030001 [doi]
PST - ppublish
SO  - Int J Environ Res Public Health. 2006 Mar;3(1):4-10. doi: 
      10.3390/ijerph2006030001.