PMID- 16823892
OWN - NLM
STAT- MEDLINE
DCOM- 20060929
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 210
IP  - 1
DP  - 2006 Sep
TI  - Dual-colour chromogenic in situ hybridization for testing of HER-2 oncogene 
      amplification in archival breast tumours.
PG  - 3-9
AB  - Chromogenic in situ hybridization (CISH), which uses an enzymatic reaction to 
      detect the hybridized DNA probe, is a new alternative to fluorescence in situ 
      hybridization (FISH) for the assessment of HER-2 oncogene amplification status in 
      breast cancer. The main advantage of CISH over FISH is the use of bright-field 
      microscopy, which is rapid and allows the histopathological evaluation of tumour 
      tissue sections. The main disadvantage of CISH has been the use of a single 
      probe, thereby making it necessary to hybridize the control probe (chromosome 17 
      centromere) on an adjacent tissue section. The present paper presents an 
      efficient protocol for dual-colour CISH (dc-CISH) based on the co-hybridization 
      of probes to the HER-2 oncogene and chromosome 17 centromere. The probes were 
      detected sequentially with antibodies to digoxigenin and biotin and with 
      secondary antibody polymers labelled with horseradish peroxidase and alkaline 
      phosphatase. The peroxidase reaction was visualized with tetramethyl benzidine 
      (green reaction product) and the alkaline phosphatase reaction with New Fuchsin 
      (red reaction product). The accuracy of the method was verified by comparing the 
      results for four cell lines and 40 tumour samples with those obtained using FISH 
      (Vysis Inc.). The results of FISH and dc-CISH showed high concordance (91%, Kappa 
      coefficient = 0.82). It is concluded that dual-colour CISH, which is a new 
      alternative to FISH enables the assessment of copy number ratio (HER-2/17 
      centromere) in conjunction with proper histopathological evaluation and the ease 
      of bright-field microscopy.
CI  - Copyright (c) 2006 Pathological Society of Great Britain and Ireland. Published 
      by John Wiley & Sons, Ltd.
FAU - Laakso, M
AU  - Laakso M
AD  - Department of Pathology, Seinajoki Central Hospital, Hanneksenrinne 7, 60220 
      Seinajoki, Finland. mervi.laakso@epshp.fi
FAU - Tanner, M
AU  - Tanner M
FAU - Isola, J
AU  - Isola J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
SB  - IM
CIN - J Pathol. 2006 Sep;210(1):1-2. PMID: 16841374
MH  - Breast Neoplasms/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Centromere/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - *Color
MH  - Female
MH  - Gene Amplification/genetics
MH  - Genes, erbB-2/*genetics
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/methods
EDAT- 2006/07/11 09:00
MHDA- 2006/09/30 09:00
CRDT- 2006/07/11 09:00
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2006/09/30 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
AID - 10.1002/path.2022 [doi]
PST - ppublish
SO  - J Pathol. 2006 Sep;210(1):3-9. doi: 10.1002/path.2022.