PMID- 16825295
OWN - NLM
STAT- MEDLINE
DCOM- 20070629
LR  - 20220331
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 575
IP  - Pt 3
DP  - 2006 Sep 15
TI  - Suppression of hippocampal plasticity-related gene expression by sleep 
      deprivation in rats.
PG  - 807-19
AB  - Previous work shows that sleep deprivation impairs hippocampal-dependent learning 
      and long-term potentiation (LTP). Brain-derived neurotrophic factor (BDNF), cAMP 
      response-element-binding (CREB) and calcium-calmodulin-dependent protein kinase 
      II (CAMKII) are critical modulators of hippocampal-dependent learning and LTP. In 
      the present study we compared the effects of short- (8 h) and intermediate-term 
      (48 h) sleep deprivation (SD) on the expression of BDNF and its downstream 
      targets, Synapsin I, CREB and CAMKII in the neocortex and the hippocampus. Rats 
      were sleep deprived using an intermittent treadmill system which equated total 
      movement in the SD and control treadmill animals (CT), but permitted sustained 
      periods of rest in CT animals. Animals were divided into SD (treadmill schedule: 
      3 s on/12 s off) and two treadmill control groups, CT1 (15 min on/60 min off) and 
      CT2 (30 min on/120 min off - permitting more sustained sleep). Real-time Taqman 
      RT-PCR was used to measure changes in mRNA; BDNF protein levels were determined 
      using ELISA. In the hippocampus, 8 h treatments reduced BDNF, Synapsin I, CREB 
      and CAMKII gene expression in both SD and control groups. Following 48 h of 
      experimental procedures, the expression of all these four molecular markers of 
      plasticity was reduced in SD and CT1 groups compared to the CT2 and cage control 
      groups. In the hippocampus, BDNF protein levels after 8 h and 48 h treatments 
      paralleled the changes in mRNA. In neocortex, neither 8 h nor 48 h SD or control 
      treatments had significant effects on BDNF, Synapsin I and CAMKII mRNA levels. 
      Stepwise regression analysis suggested that loss of REM sleep underlies the 
      effects of SD on hippocampal BDNF, Synapsin I and CREB mRNA levels, whereas loss 
      of NREM sleep underlies the effects on CAMKII mRNA.
FAU - Guzman-Marin, Ruben
AU  - Guzman-Marin R
AD  - Research Service (151A3), V. A. Greater Los Angeles Healthcare System, 16111 
      Plummer Street, North Hills CA 91343, USA.
FAU - Ying, Zhe
AU  - Ying Z
FAU - Suntsova, Natalia
AU  - Suntsova N
FAU - Methippara, Melvi
AU  - Methippara M
FAU - Bashir, Tariq
AU  - Bashir T
FAU - Szymusiak, Ronald
AU  - Szymusiak R
FAU - Gomez-Pinilla, Fernando
AU  - Gomez-Pinilla F
FAU - McGinty, Dennis
AU  - McGinty D
LA  - eng
GR  - P50 HL060296/HL/NHLBI NIH HHS/United States
GR  - R01 MH047480/MH/NIMH NIH HHS/United States
GR  - HL 60296/HL/NHLBI NIH HHS/United States
GR  - MH 47480/MH/NIMH NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060706
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Synapsins)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/genetics/metabolism
MH  - Cyclic AMP Response Element-Binding Protein/genetics/metabolism
MH  - *Gene Expression Regulation
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Models, Animal
MH  - Neocortex/metabolism
MH  - Neuronal Plasticity/*genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sleep Deprivation/*genetics/metabolism
MH  - Synapsins/genetics/metabolism
MH  - Time Factors
MH  - Wakefulness/genetics
PMC - PMC1995680
EDAT- 2006/07/11 09:00
MHDA- 2007/06/30 09:00
PMCR- 2007/09/15
CRDT- 2006/07/11 09:00
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2007/06/30 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
PHST- 2007/09/15 00:00 [pmc-release]
AID - jphysiol.2006.115287 [pii]
AID - 10.1113/jphysiol.2006.115287 [doi]
PST - ppublish
SO  - J Physiol. 2006 Sep 15;575(Pt 3):807-19. doi: 10.1113/jphysiol.2006.115287. Epub 
      2006 Jul 6.