PMID- 16825452
OWN - NLM
STAT- MEDLINE
DCOM- 20061205
LR  - 20060707
IS  - 0047-2425 (Print)
IS  - 0047-2425 (Linking)
VI  - 35
IP  - 4
DP  - 2006 Jul-Aug
TI  - Microbial populations identified by fluorescence in situ hybridization in a 
      constructed wetland treating acid coal mine drainage.
PG  - 1329-37
AB  - Microorganisms are an integral part of the biogeochemical processes in wetlands, 
      yet microbial communities in sediments within constructed wetlands receiving acid 
      mine drainage (AMD) are only poorly understood. The purpose of this study was to 
      characterize the microbial diversity and abundance in a wetland receiving AMD 
      using fluorescence in situ hybridization (FISH) analysis. Seasonal samples of 
      oxic surface sediments, comprised of Fe(III) precipitates, were collected from 
      two treatment cells of the constructed wetland system. The pH of the bulk samples 
      ranged between pH 2.1 and 3.9. Viable counts of acidophilic Fe and S oxidizers 
      and heterotrophs were determined with a most probable number (MPN) method. The 
      MPN counts were only a fraction of the corresponding FISH counts. The sediment 
      samples contained microorganisms in the Bacteria (including the subgroups of 
      acidophilic Fe- and S-oxidizing bacteria and Acidiphilium spp.) and Eukarya 
      domains. Archaea were present in the sediment surface samples at < 0.01% of the 
      total microbial community. The most numerous bacterial species in this wetland 
      system was Acidithiobacillus ferrooxidans, comprising up to 37% of the bacterial 
      population. Acidithiobacillus thiooxidans was also abundant. Heterotrophs in the 
      Acidiphilium genus totaled 20% of the bacterial population. Leptospirillum 
      ferrooxidans was below the level of detection in the bacterial community. The 
      results from the FISH technique from this field study are consistent with results 
      from other experiments involving enumeration by most probable number, dot-blot 
      hybridization, and denaturing gradient gel electrophoresis analyses and with the 
      geochemistry of the site.
FAU - Nicomrat, Duongruitai
AU  - Nicomrat D
AD  - Environmental Science Graduate Program, The Ohio State University, Columbus, OH 
      43210, USA.
FAU - Dick, Warren A
AU  - Dick WA
FAU - Tuovinen, Olli H
AU  - Tuovinen OH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060706
PL  - United States
TA  - J Environ Qual
JT  - Journal of environmental quality
JID - 0330666
RN  - 0 (Acids)
SB  - IM
MH  - Acidithiobacillus/drug effects/growth & development
MH  - Acids/*pharmacology
MH  - Bacteria/classification/*drug effects/growth & development
MH  - Base Sequence
MH  - *Coal Mining
MH  - Ecosystem
MH  - Environmental Microbiology
MH  - Geologic Sediments/*microbiology
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - Population Dynamics
MH  - *Water Microbiology
EDAT- 2006/07/11 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/07/11 09:00
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
AID - 35/4/1329 [pii]
AID - 10.2134/jeq2005.0325 [doi]
PST - epublish
SO  - J Environ Qual. 2006 Jul 6;35(4):1329-37. doi: 10.2134/jeq2005.0325. Print 2006 
      Jul-Aug.