PMID- 16825771 OWN - NLM STAT- MEDLINE DCOM- 20060728 LR - 20060707 IS - 1424-859X (Electronic) IS - 1424-8581 (Linking) VI - 114 IP - 2 DP - 2006 TI - Differentiating sex chromosomes of the dioecious Spinacia oleracea L. (spinach) by FISH of 45S rDNA. PG - 175-7 AB - Spinacia oleracea L. (spinach) is a dioecious species with both male and female plants having 2n = 2x = 12 chromosomes, consisting of two large metacentrics, two long subtelocentrics, two short subtelocentrics, two acrocentrics, and four submetacentrics. The location of 45S rDNA was investigated on metaphase chromosomes using fluorescence in situ hybridization (FISH). The numbers of 45S rDNA foci in diploid sets of chromosomes from females was six and from males was five. All the fluorescent foci lay in secondary constrictions and the satellites. Our results indicate that an XY-type sex chromosome system could be present in spinach where the Y chromosome lacks a 45S RNA focus. CI - Copyright (c) 2006 S. Karger AG, Basel. FAU - Lan, T AU - Lan T AD - Laboratory of Chromosome Research, College of Life Sciences, Nankai University, Tianjin, PR China. FAU - Zhang, S AU - Zhang S FAU - Liu, B AU - Liu B FAU - Li, X AU - Li X FAU - Chen, R AU - Chen R FAU - Song, W AU - Song W LA - eng PT - Journal Article PL - Switzerland TA - Cytogenet Genome Res JT - Cytogenetic and genome research JID - 101142708 RN - 0 (DNA, Plant) RN - 0 (DNA, Ribosomal) RN - 0 (RNA, Ribosomal) RN - 0 (RNA, ribosomal, 45S) SB - IM MH - Chromosomes, Plant/*genetics MH - DNA, Plant/*genetics MH - DNA, Ribosomal/*genetics MH - *In Situ Hybridization, Fluorescence MH - Karyotyping MH - RNA, Ribosomal/*genetics MH - Sex Chromosomes/*genetics MH - Spinacia oleracea/*genetics EDAT- 2006/07/11 09:00 MHDA- 2006/07/29 09:00 CRDT- 2006/07/11 09:00 PHST- 2005/08/17 00:00 [received] PHST- 2005/11/29 00:00 [accepted] PHST- 2006/07/11 09:00 [pubmed] PHST- 2006/07/29 09:00 [medline] PHST- 2006/07/11 09:00 [entrez] AID - 93335 [pii] AID - 10.1159/000093335 [doi] PST - ppublish SO - Cytogenet Genome Res. 2006;114(2):175-7. doi: 10.1159/000093335.