PMID- 16826913 OWN - NLM STAT- MEDLINE DCOM- 20100518 LR - 20131121 IS - 1000-0593 (Print) IS - 1000-0593 (Linking) VI - 26 IP - 2 DP - 2006 Feb TI - [Fluorescence resonance energy transfer quenching method for determination of arsenic with acridine orange-rhodamine B]. PG - 306-8 AB - A new method for the determination of trace arsenic was developed by using fluorescence resonance energy transfer from acridine orange (AO) to rhodamine B (RB). It was found that under the condition of lambda(ex)/lambda(em) = 470/580 nm, effective energy transfer could occur between AO and RB in the dodecyl benzene sodium sulfonate solution. The fluorescence intensity of RB was diminished by molybdoarsenide which was formed by the reaction of arsenic (V) with molybdate in sulfuric acid medium. The detection limit of this method was 2. 56 microg x L(-1). This method was used for the determination of trace arsenic in tea. The range of determination for arsenic was 0.01-0.25 mg x L(-1). The relative standard deviation for the determination of arsenic was 0.48%-0.64%. The recoveries for the addition of 0.01-0.03 mg x L(-1) arsenic were 98%-103%. The method has been applied to the determination of arsenic with satisfactory results. FAU - Liu, Bao-Sheng AU - Liu BS AD - The Center of Physics and Chemistry, Hebei Provincial Key Laboratory of Analytical Science and Technology, Hebei University, Baoding 071002, China. FAU - Gao, Jing AU - Gao J FAU - Liu, Zhi-Chao AU - Liu ZC FAU - Yu, Li-Na AU - Yu LN FAU - Yang, Da-Cheng AU - Yang DC FAU - Yang, Geng-Liang AU - Yang GL LA - chi PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Guang Pu Xue Yu Guang Pu Fen Xi JT - Guang pu xue yu guang pu fen xi = Guang pu JID - 9424805 RN - 0 (Fluorescent Dyes) RN - 0 (Rhodamines) RN - F30N4O6XVV (Acridine Orange) RN - K7G5SCF8IL (rhodamine B) RN - N712M78A8G (Arsenic) SB - IM MH - Acridine Orange/chemistry MH - Arsenic/*analysis MH - Camellia sinensis/*chemistry MH - Fluorescence Resonance Energy Transfer/instrumentation/*methods MH - Fluorescent Dyes/chemistry MH - Plant Leaves/chemistry MH - Rhodamines/chemistry EDAT- 2006/07/11 09:00 MHDA- 2010/05/19 06:00 CRDT- 2006/07/11 09:00 PHST- 2006/07/11 09:00 [pubmed] PHST- 2010/05/19 06:00 [medline] PHST- 2006/07/11 09:00 [entrez] PST - ppublish SO - Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Feb;26(2):306-8.