PMID- 16827115
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20181201
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 26
IP  - 3A
DP  - 2006 May-Jun
TI  - Vascular endothelial growth factor and dendritic cells in human squamous cell 
      carcinoma of the oral cavity.
PG  - 1833-48
AB  - Dendritic cells (DCs) play an important role in the host immune defense against 
      tumors, and there is an inverse correlation between DC density and the expression 
      of vascular endothelial growth factor (VEGF). However, the relationship between 
      VEGF expression in tumors and infiltration of CD1a+ or CD83+ DCs, which express 
      the VEGF receptor (VEGFR), remains unclear. Therefore, in vivo and in vitro 
      studies were conducted to investigate the relationship between VEGF expression 
      and DC subsets in oral squamous cell carcinomas (OSCCs). Strong VEGF expression 
      was detected in cancer tissues from patients with regional lymph node metastasis 
      (PN+ cases). In these tissues, the VEGF expression correlated inversely with the 
      number of CD1a + DCs, but positively with the number of CD83+ DCs. Large amounts 
      of VEGF were secreted by OSCCs cell lines, and their culture supernatants 
      significantly inhibited the production of differentiated CD1a+ DCs from 
      peripheral blood mononuclear cells (PBMCs), whereas differentiated CD83+ DCs were 
      increased. VEGFR-1 and -2 were detected in a few PBMCs and CD1a+ DCs. 
      Furthermore, CD1a mRNA disappeared when recombinant human VEGF165 (rhVEGF165) was 
      added to CD1a+ DCs, while CD83 mRNA increased. These results suggest that, in 
      OSCCs, secreted VEGF might promote escape from tumor immunity by inhibiting the 
      differentiation of CD1a + DCs from progenitor cells and increasing the levels of 
      dysfunctional CD83+ DCs.
FAU - Kikuchi, Kentaro
AU  - Kikuchi K
AD  - Department of Pathology, Nihon University School of Medicine, 30-1 Oyaguchi 
      Kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. kentak@med.nihon-u.ac.jp
FAU - Kusama, Kaoru
AU  - Kusama K
FAU - Sano, Makoto
AU  - Sano M
FAU - Nakanishi, Yoko
AU  - Nakanishi Y
FAU - Ishige, Toshiyuki
AU  - Ishige T
FAU - Ohni, Sumie
AU  - Ohni S
FAU - Oinuma, Toshinori
AU  - Oinuma T
FAU - Nemoto, Norimichi
AU  - Nemoto N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1a antigen)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD1/immunology
MH  - Carcinoma, Squamous Cell/*immunology/*metabolism
MH  - Cell Differentiation/drug effects/immunology
MH  - Cell Line, Tumor
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mouth Neoplasms/*immunology/*metabolism
MH  - Vascular Endothelial Growth Factor A/*biosynthesis/metabolism/pharmacology
EDAT- 2006/07/11 09:00
MHDA- 2006/08/25 09:00
CRDT- 2006/07/11 09:00
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2006 May-Jun;26(3A):1833-48.