PMID- 16827307
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 1
DP  - 2006 Jan
TI  - [Determination of organic acids in cane vinasse by micellar electrokinetic 
      capillary chromatography with indirect ultraviolet detection].
PG  - 35-8
AB  - Micellar electrokinetic capillary chromatography (MECC) with indirect ultraviolet 
      (UV) detection method for the separation and determination of several organic 
      acids in cane vinasse, including malonic, formic, tartaric, malic, succinic, 
      glutaric, acetic, lactic and glutamic acids, were developed. Electrophoretic 
      conditions were as follows: uncoated fused silica capillary (56 cm/ 64 cm 
      (effective/total length), 50 microm i. d. ), 7.5 mmol/L potassium acid 
      phthalate-1. 5 mmol/L cetyltrimethyl-ammonium bromide (CTAB) at pH = 6.50 as 
      buffer solution, applied voltage -25 kV, temperature 25 degrees C, detection 
      wavelength 300 nm, reference wavelength 210 nm. Good linearities were obtained 
      for nine organic acids, and the detection limits were 0.5 mg/L, 0.3 mg/L, 1.5 
      mg/L, 1.5 mg/L, 0.3 mg/L, 0.3 mg/L, 0.4 mg/L, 0.4 mg/L, 0.4 mg/L for malonic, 
      formic, tartaric, malic, succinic, glutaric, acetic, lactic and glutamic acid, 
      respectively. The relative standard deviations (RSDs) for migration times and 
      peak areas of nine organic acids within a day were 0.4% - 0.6% and 2.3% - 4.8%, 
      respectively. The corresponding data for five days were 0.5% -0.7% and 3.3% - 
      5.2%. The recoveries of acid standards were above 93%. The method can be applied 
      to determine the organic acids in cane vinasse with satisfactory results.
FAU - Xu, Yuanjin
AU  - Xu Y
AD  - Engineering Center For Sugar Cane and Cane Sugar, Guangxi University, Nanning 
      530004, China. yjxu@gxu.edu.cn
FAU - Xu, Guiping
AU  - Xu G
FAU - Wei, Yuanan
AU  - Wei Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Acids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Organic Chemicals)
RN  - 0 (Phthalic Acids)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Acids/*analysis
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Drugs, Chinese Herbal/chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Limit of Detection
MH  - Molasses/analysis
MH  - Organic Chemicals
MH  - Phthalic Acids/analysis
MH  - Plant Extracts/*chemistry
MH  - Saccharum/*chemistry
EDAT- 2006/07/11 09:00
MHDA- 2010/07/08 06:00
CRDT- 2006/07/11 09:00
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 Jan;24(1):35-8.