PMID- 16829164
OWN - NLM
STAT- MEDLINE
DCOM- 20061102
LR  - 20061115
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 608
IP  - 2
DP  - 2006 Sep 28
TI  - Cytogenetic effects in children and mothers exposed to air pollution assessed by 
      the frequency of micronuclei and fluorescence in situ hybridization (FISH): a 
      family pilot study in the Czech Republic.
PG  - 112-20
AB  - A family pilot study was conducted in the Czech Republic to test the hypothesis 
      that exposure to air pollution with particulate matter (PM) in children results 
      in detectable effects indicated by a number of biomarkers of exposure and early 
      effects. The frequency of micronuclei (MN) in peripheral blood lymphocytes (PBLs) 
      was analysed to assess the cytogenetic effects in children and mothers living in 
      two different areas. From each area two groups of children from a total of 24 
      families (mean age: 6.0+/-0.6 and 9.0+/-1.2 years) in a total of 47 children and 
      19 mothers (mean age: 33.6+/-3.9 years) participated. Chromosome aberrations 
      determined with fluorescence in situ hybridization (FISH) painting for 
      chromosomes #1 and #4 were analysed in 39 children and 20 parents. Teplice, a 
      mining district, in Northern Bohemia was selected for the analyses of the effects 
      in a population exposed to high levels of air pollution, especially during 
      winter, and compared with a population from the rural area of Prachatice in 
      Southern Bohemia. Significant higher frequencies of MN were found in the younger 
      children living in the Teplice area as compared with those living in the 
      Prachatice area (7.0+/-2.3 per thousand versus 4.9+/-2.0 per thousand, p=0.04). 
      Higher levels of MN were also measured in the older children and the mothers from 
      the Teplice area (9.2+/-3.7 per thousand versus 6.6+/-4.4 per thousand) and 
      (12.6+/-3.4 per thousand versus 10.1+/-4.0 per thousand). The increased MN 
      frequency may be associated with elevated carcinogenic polycyclic aromatic 
      hydrocarbons (c-PAHs) concentration of the PM(2.5) measured in the ambient 
      Teplice air, but other factors like genotoxic compounds from the diet or 
      protective effect of micronutrients, which was not addressed in this pilot study, 
      may also differ between the two areas. MN frequencies were found to increase with 
      age in children. Lower MN frequency was found in boys as compared to girls. The 
      result of the FISH analyses showed a low number of individuals with detectable 
      levels of aberrations and no significant increases in genomic frequency of stable 
      chromosome exchanges (F(G)/100) were found in children or parents from the 
      Teplice area in comparison with those from the Prachatice area. The family pilot 
      study indicates that MN is a valuable and sensitive biomarker for early 
      biological effect in children and adults living in two different areas 
      characterised with significant exposure differences in c-PAHs concentrations 
      during winter.
FAU - Pedersen, Marie
AU  - Pedersen M
AD  - Institute of Public Health, Department of Environmental and Occupational Health, 
      University of Copenhagen, DK-1014 K Copenhagen, Denmark.
FAU - Vinzents, Peter
AU  - Vinzents P
FAU - Petersen, Joergen Holm
AU  - Petersen JH
FAU - Kleinjans, Jos C S
AU  - Kleinjans JC
FAU - Plas, Gina
AU  - Plas G
FAU - Kirsch-Volders, Micheline
AU  - Kirsch-Volders M
FAU - Dostal, Miroslav
AU  - Dostal M
FAU - Rossner, Pavel
AU  - Rossner P
FAU - Beskid, Olena
AU  - Beskid O
FAU - Sram, Radim J
AU  - Sram RJ
FAU - Merlo, Domenico Franco
AU  - Merlo DF
FAU - Knudsen, Lisbeth Ehlert
AU  - Knudsen LE
LA  - eng
PT  - Journal Article
DEP - 20060707
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Air Pollutants)
SB  - IM
MH  - Adult
MH  - Air Pollutants/toxicity
MH  - Air Pollution/*adverse effects
MH  - Child
MH  - Child, Preschool
MH  - Cytogenetics
MH  - Czech Republic
MH  - Environmental Exposure
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Micronuclei, Chromosome-Defective/chemically induced
MH  - Micronucleus Tests
MH  - Pilot Projects
MH  - Siblings
EDAT- 2006/07/11 09:00
MHDA- 2006/11/03 09:00
CRDT- 2006/07/11 09:00
PHST- 2005/11/04 00:00 [received]
PHST- 2006/02/14 00:00 [revised]
PHST- 2006/02/15 00:00 [accepted]
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2006/11/03 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
AID - S1383-5718(06)00149-5 [pii]
AID - 10.1016/j.mrgentox.2006.02.013 [doi]
PST - ppublish
SO  - Mutat Res. 2006 Sep 28;608(2):112-20. doi: 10.1016/j.mrgentox.2006.02.013. Epub 
      2006 Jul 7.