PMID- 16830212
OWN - NLM
STAT- MEDLINE
DCOM- 20061003
LR  - 20181113
IS  - 0364-3190 (Print)
IS  - 0364-3190 (Linking)
VI  - 31
IP  - 7
DP  - 2006 Jul
TI  - Reversed actrocytic GLT-1 during ischemia is crucial to excitotoxic death of 
      neurons, but contributes to the survival of astrocytes themselves.
PG  - 933-43
AB  - During ischemia, the operation of astrocytic/neuronal glutamate transporters is 
      reversed and glutamate and Na(+) are co-transported to the extracellular space. 
      This study aims to investigate whether this reversed operation of glutamate 
      transporters has any functional meanings for astrocytes themselves. 
      Oxygen/glucose deprivation (OGD) of neuron/astrocyte co-cultures resulted in the 
      massive death of neurons, and the cell death was significantly reduced by 
      treatment with either AP5 or DHK. In cultured astrocytes with little GLT-1 
      expression, OGD produced Na(+) overload, resulting in the reversal of astrocytic 
      Na(+)/Ca(2+)-exchanger (NCX). The reversed NCX then caused Ca(2+) overload 
      leading to the damage of astrocytes. In contrast, the OGD-induced Na(+) overload 
      and astrocytic damage were significantly attenuated in PACAP-treated astrocytes 
      with increased GLT-1 expression, and the attenuation was antagonized by treatment 
      with DHK. These results suggested that the OGD-induced reversal of GLT-1 
      contributed to the survival of astrocytes themselves by releasing Na(+) with 
      glutamate via reversed GLT-1.
FAU - Kosugi, Tatsuro
AU  - Kosugi T
AD  - Laboratory of Cellular Cybernetics, Graduate School of Information Science and 
      Technology, Hokkaido University, Sapporo 060-0814, Japan.
FAU - Kawahara, Koichi
AU  - Kawahara K
LA  - eng
PT  - Journal Article
DEP - 20060708
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Excitatory Amino Acid Transporter 2)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Brain Ischemia/*metabolism
MH  - *Cell Survival
MH  - Coculture Techniques
MH  - Excitatory Amino Acid Transporter 2/*metabolism
MH  - Immunohistochemistry
MH  - Neurons/metabolism
MH  - Rats
EDAT- 2006/07/11 09:00
MHDA- 2006/10/04 09:00
CRDT- 2006/07/11 09:00
PHST- 2006/06/05 00:00 [accepted]
PHST- 2006/07/11 09:00 [pubmed]
PHST- 2006/10/04 09:00 [medline]
PHST- 2006/07/11 09:00 [entrez]
AID - 10.1007/s11064-006-9099-6 [doi]
PST - ppublish
SO  - Neurochem Res. 2006 Jul;31(7):933-43. doi: 10.1007/s11064-006-9099-6. Epub 2006 
      Jul 8.