PMID- 16831829
OWN - NLM
STAT- MEDLINE
DCOM- 20061214
LR  - 20181113
IS  - 0003-4967 (Print)
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 65
IP  - 12
DP  - 2006 Dec
TI  - Signs of inflammation in both symptomatic and asymptomatic muscles from patients 
      with polymyositis and dermatomyositis.
PG  - 1565-71
AB  - OBJECTIVES: To determine whether muscle weakness is correlated with inflammation, 
      expression of interleukin 1alpha (IL1alpha) and major histocompatibility complex 
      (MHC) class I and II antigens on muscle fibres. METHODS: Biopsy specimens from 
      clinically symptomatic (proximal muscles) and asymptomatic (all distal but two 
      proximal) muscles in eight patients with polymyositis, three patients with 
      dermatomyositis and six healthy controls were analysed by immunohistochemistry 
      for the presence of T cells and macrophages, and expression of IL1alpha and of 
      MHC class I and II antigens. RESULTS: were evaluated by conventional light 
      microscopy and by computerised image analysis. Results: Inflammatory infiltrates 
      with T cells and macrophages were observed to an equal degree in both symptomatic 
      and asymptomatic muscle. The numbers of capillaries with IL1alpha expression were 
      significantly higher (p<0.05) in the symptomatic and asymptomatic muscles of 
      patients than in controls. The total IL1alpha expression per tissue section 
      assessed by computerised image analysis was significantly higher in symptomatic 
      muscles but not in asymptomatic muscles compared with that in controls. Neither 
      the number of IL1alpha-positive capillaries nor the total IL1alpha expression 
      differed significantly between symptomatic and asymptomatic muscles. Expression 
      of MHC class I and II antigens on muscle fibres was detected in both symptomatic 
      and asymptomatic muscles but rarely in healthy controls. CONCLUSIONS: Presence of 
      inflammatory infiltrates, T cells and macrophages, and expression of MHC class I 
      and II antigens and of IL1alpha on muscle fibres were independent of clinical 
      symptoms, and were present to an equal degree in both proximal and distal 
      muscles. Thus, other factors seem to determine the development of clinical 
      symptoms. One such factor could be variations in physical demands.
FAU - Dorph, C
AU  - Dorph C
AD  - Rheumatology Unit, Department of Medicine, Karolinska University Hospital, 
      Karolinska Institutet, Solna, Sweden. Christina.Dorph@karolinska.se
FAU - Englund, P
AU  - Englund P
FAU - Nennesmo, I
AU  - Nennesmo I
FAU - Lundberg, I E
AU  - Lundberg IE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060710
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (CD3 Complex)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Interleukin-1alpha)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Biopsy
MH  - CD3 Complex/metabolism
MH  - Dermatomyositis/immunology/pathology
MH  - Female
MH  - HLA-DR Antigens/metabolism
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Humans
MH  - Interleukin-1alpha/metabolism
MH  - Macrophages/pathology
MH  - Male
MH  - Middle Aged
MH  - Muscle Fibers, Skeletal/immunology/pathology
MH  - Muscle, Skeletal/immunology/*pathology
MH  - Polymyositis/immunology/*pathology
MH  - Receptors, Cell Surface/metabolism
MH  - T-Lymphocyte Subsets/pathology
PMC - PMC1798445
COIS- Competing interests: None.
EDAT- 2006/07/13 09:00
MHDA- 2006/12/15 09:00
PMCR- 2009/12/01
CRDT- 2006/07/13 09:00
PHST- 2006/07/13 09:00 [pubmed]
PHST- 2006/12/15 09:00 [medline]
PHST- 2006/07/13 09:00 [entrez]
PHST- 2009/12/01 00:00 [pmc-release]
AID - ard.2005.051086 [pii]
AID - ar51086 [pii]
AID - 10.1136/ard.2005.051086 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2006 Dec;65(12):1565-71. doi: 10.1136/ard.2005.051086. Epub 2006 
      Jul 10.