PMID- 1683237
OWN - NLM
STAT- MEDLINE
DCOM- 19911230
LR  - 20181130
IS  - 0904-1850 (Print)
IS  - 0904-1850 (Linking)
VI  - 13
DP  - 1991 Apr
TI  - Regulation of human IgE synthesis: the role of CD4+ and CD8+ T-cells and the 
      inhibitory effects of interferon-alpha.
PG  - 31s-38s
AB  - Interleukin-4 (IL-4) has been shown to induce immunoglobulin E (IgE) synthesis by 
      human peripheral blood lymphocytes (PBL). Here we show that highly purified 
      B-cells (greater than 99.5%) failed to produce IgE in the presence of IL-4 but 
      IgE synthesis was restored by the addition of autologous CD4+ T-cells or by CD4+ 
      T-cell clones with non-relevant specificities, derived from different donors. In 
      contrast, autologous CD8+ T-lymphocytes or one allogeneic CD8+ T-cell clone 
      failed to restore IgE synthesis. Moreover, autologous CD8+ T-cells suppressed IgE 
      synthesis induced by autologous CD4+ T-cells in a dose-dependent fashion. IgE 
      production could be induced in cultures containing as few as 20 B-cells. 
      Collectively these data indicate that in addition to IL-4, a second signal 
      derived from CD4+ T-cells is required to induce B-cells to switch to IgE 
      producing cells. In a second set of experiments we showed that IFN-alpha blocked 
      both IL-4-induced IgE synthesis by PBL of healthy donors and spontaneous IgE 
      synthesis by PBL of allergic or atopic patients in a dose-dependent fashion. This 
      inhibition occurred at the IgE messenger ribonucleic acid (mRNA) transcription 
      level. The strongest inhibitory effects of interferon-alpha (IFN-alpha) were 
      observed at the 2.2 kb productive mRNA transcript, whereas weaker inhibitory 
      effects were observed on the 1.7 kb germline IgE mRNA transcript.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Gauchat, J F
AU  - Gauchat JF
AD  - DNAX Research Institute, Palo Alto, CA 94304-1104.
FAU - Gascan, H
AU  - Gascan H
FAU - Roncarolo, M G
AU  - Roncarolo MG
FAU - Rousset, F
AU  - Rousset F
FAU - Pene, J
AU  - Pene J
FAU - de Vries, J E
AU  - de Vries JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur Respir J Suppl
JT  - The European respiratory journal. Supplement
JID - 8910681
RN  - 0 (Interferon alpha-2)
RN  - 0 (Interferon-alpha)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - B-Lymphocytes/drug effects/*metabolism
MH  - CD4-Positive T-Lymphocytes/physiology
MH  - Clone Cells
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis/genetics
MH  - Interferon alpha-2
MH  - Interferon-alpha/*pharmacology
MH  - Interleukin-4/*physiology
MH  - Recombinant Proteins
MH  - T-Lymphocytes/*physiology
MH  - T-Lymphocytes, Cytotoxic/physiology
MH  - T-Lymphocytes, Regulatory/physiology
MH  - Transcription, Genetic/drug effects
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
PST - ppublish
SO  - Eur Respir J Suppl. 1991 Apr;13:31s-38s.