PMID- 16832647
OWN - NLM
STAT- MEDLINE
DCOM- 20061019
LR  - 20220408
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 113
IP  - 4
DP  - 2006 Aug
TI  - Genome constitution and evolution in Lolium x Festuca hybrid cultivars 
      (Festulolium).
PG  - 731-42
AB  - Festulolium hybrids are being increasingly used worldwide as forage grasses. This 
      is due to their superior agronomic characteristics, which combine yield 
      performance of ryegrasses (Lolium multiflorum and L. perenne) and tolerance 
      against abiotic stress of fescues (Festuca pratensis, F. arundinacea and F. 
      arundinacea var. glaucescens). Despite the widespread use, only fragmentary 
      information exists on their genomic constitution. We used genomic in situ 
      hybridization (GISH) to analyze genomic constitution of over 600 plants from 
      almost all commercially available cultivars of Festulolium. Our results revealed 
      a surprisingly large range of variation in the proportions of parental genomes 
      and in the extent of intergenomic recombination. Using fluorescence in situ 
      hybridization (FISH) with probes for ribosomal DNA, we assessed the frequency of 
      recombination and elimination of particular chromosomes and chromosome groups in 
      three contrasting Festulolium cultivars. This study provides novel information 
      that will aid in understanding the relationship between a genetic make-up and the 
      phenotype of Festulolium hybrids. Our results indicate that GISH might be a 
      useful tool to aid in Festulolium breeding and provide data for a more detailed 
      description of registered cultivars.
FAU - Kopecky, D
AU  - Kopecky D
AD  - Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental 
      Botany, Sokolovska 6, 77200 Olomouc, Czech Republic. kopecky@ueb.cas.cz
FAU - Loureiro, J
AU  - Loureiro J
FAU - Zwierzykowski, Z
AU  - Zwierzykowski Z
FAU - Ghesquiere, M
AU  - Ghesquiere M
FAU - Dolezel, J
AU  - Dolezel J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060711
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
SB  - IM
MH  - Chromosomes, Plant
MH  - *Evolution, Molecular
MH  - Festuca/*genetics
MH  - *Genome, Plant
MH  - *Hybridization, Genetic
MH  - In Situ Hybridization
MH  - Karyotyping
MH  - Lolium/*genetics
MH  - Recombination, Genetic
EDAT- 2006/07/13 09:00
MHDA- 2006/10/20 09:00
CRDT- 2006/07/13 09:00
PHST- 2006/02/21 00:00 [received]
PHST- 2006/06/03 00:00 [accepted]
PHST- 2006/07/13 09:00 [pubmed]
PHST- 2006/10/20 09:00 [medline]
PHST- 2006/07/13 09:00 [entrez]
AID - 10.1007/s00122-006-0341-z [doi]
PST - ppublish
SO  - Theor Appl Genet. 2006 Aug;113(4):731-42. doi: 10.1007/s00122-006-0341-z. Epub 
      2006 Jul 11.