PMID- 16832844
OWN - NLM
STAT- MEDLINE
DCOM- 20061128
LR  - 20231213
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 33
IP  - 8
DP  - 2006 Aug
TI  - Avocado/soybean unsaponifiables prevent the inhibitory effect of osteoarthritic 
      subchondral osteoblasts on aggrecan and type II collagen synthesis by 
      chondrocytes.
PG  - 1668-78
AB  - OBJECTIVE: To determine the effects of avocado/soybean unsaponifiables (ASU) on 
      osteoblast-induced dysregulation of chondrocyte metabolism. METHODS: Human 
      chondrocytes were isolated from osteoarthritis (OA) cartilage and cultured in 
      alginate beads for 4 or 10 days in the absence or presence of osteoblasts 
      isolated from nonsclerotic (NSC) or sclerotic (SC) zones of OA subchondral bone 
      plate in monolayer. Before co-culture, osteoblasts were incubated or not with 10 
      microg/ml ASU for 72 hours. Aggrecan, type II collagen, matrix 
      metalloproteinase-3 (MMP-3) and MMP-13, tissue inhibitor of metalloproteinase 
      (TIMP-1), transforming growth factor-beta1 (TGF-beta1) and TGF-beta3, inducible 
      NO synthase (iNOS), and cyclooxygenase-2 (COX-2) mRNA levels in chondrocytes were 
      quantified by RT-PCR. Aggrecan, osteocalcin, TGF-beta1, interleukin 1beta 
      (IL-1beta), and IL-6 production were assayed by immunoassays. RESULTS: In 
      co-culture, SC osteoblasts induced a significant inhibition of matrix protein 
      production and a significant increase of MMP synthesis by chondrocytes. In 
      contrast, SC osteoblasts did not modify TIMP-1, TGF-beta1 and TGF-beta3, iNOS, or 
      COX-2 mRNA levels in chondrocytes. The pretreatment of SC osteoblasts with ASU 
      fully prevented the inhibitory effects of SC osteoblasts on matrix component 
      production, and even significantly increased type II collagen mRNA level over the 
      control (chondrocytes alone) value. In contrast, pretreatment of SC osteoblasts 
      with ASU did not significantly modify the expression of MMP, TIMP-1, TGF-beta1, 
      TGF-beta3, iNOS, or COX-2 gene by chondrocytes. CONCLUSION: ASU prevent the 
      osteoarthritic osteoblast-induced inhibition of matrix molecule production, 
      suggesting that this compound may promote OA cartilage repair by acting on 
      subchondral bone osteoblasts. This finding constitutes a new mechanism of action 
      for this compound, known for its beneficial effects on cartilage.
FAU - Henrotin, Yves E
AU  - Henrotin YE
AD  - Bone and Cartilage Metabolism Research Unit, Institute of Pathology B23, Liege, 
      Belgium. yhenrotin@ulg.ac.be
FAU - Deberg, Michelle A
AU  - Deberg MA
FAU - Crielaard, Jean-Michel
AU  - Crielaard JM
FAU - Piccardi, Nathalie
AU  - Piccardi N
FAU - Msika, Philippe
AU  - Msika P
FAU - Sanchez, Christelle
AU  - Sanchez C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Aggrecans)
RN  - 0 (Biomarkers)
RN  - 0 (Collagen Type II)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.- (MMP13 protein, human)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Aged
MH  - Aggrecans/*biosynthesis/genetics
MH  - Biomarkers/metabolism
MH  - Cartilage, Articular/cytology/metabolism
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/*metabolism
MH  - Coculture Techniques
MH  - Collagen Type II/*biosynthesis/genetics
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 13/biosynthesis/genetics
MH  - Matrix Metalloproteinase 3/biosynthesis/genetics
MH  - Middle Aged
MH  - Osteoarthritis, Knee/metabolism/pathology
MH  - Osteoblasts/cytology/drug effects/*metabolism
MH  - Persea/*chemistry
MH  - Plant Extracts/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Glycine max/*chemistry
EDAT- 2006/07/13 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/07/13 09:00
PHST- 2006/07/13 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/07/13 09:00 [entrez]
AID - 06/13/079 [pii]
PST - ppublish
SO  - J Rheumatol. 2006 Aug;33(8):1668-78.