PMID- 1683567
OWN - NLM
STAT- MEDLINE
DCOM- 19920106
LR  - 20191021
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 3
IP  - 4
DP  - 1991 Jul
TI  - Detection and analysis of origin of i(12p), a diagnostic marker of human male 
      germ cell tumors, by fluorescence in situ hybridization.
PG  - 300-7
AB  - The i(12p) chromosome marker has been shown to be a diagnostic and prognostic 
      marker of human male germ cell tumors (GCTs). An analysis of the i(12p) and 
      chromosome 12 aneuploidy was performed in five primary cell cultures and three 
      established cell lines derived from human male GCTs by fluorescence in situ 
      hybridization (FISH) with a chromosome 12 centromere-specific alpha-satellite DNA 
      probe. Distinct differences in the centromeric signals originating from the 
      i(12p) and normal chromosome 12 were detected, which were found to be useful for 
      unambiguous distinction between the i(12p) and normal chromosomes 12 at 
      interphase as well as at metaphase in these cultures. This method can be used for 
      rapid screening of large numbers of interphase cells, eliminating the main 
      limitation of conventional karyotypic analysis, namely, frequent inability to 
      obtain sufficient numbers of dividing cells in direct preparations or in 
      short-term culture of fresh biopsies. Our analysis of chromosome 12 centromeric 
      signal size along with karyotypic data and results of analysis of restriction 
      fragment length polymorphisms (RFLPs) on 12q in four GCTs suggested that the 
      i(12p)s are formed by nonreciprocal centromeric interchanges between nonsister 
      chromatids of homologous chromosomes.
FAU - Mukherjee, A B
AU  - Mukherjee AB
AD  - Laboratory of Cancer Genetics, Sloan-Kettering Institute, New York, New York.
FAU - Murty, V V
AU  - Murty VV
FAU - Rodriguez, E
AU  - Rodriguez E
FAU - Reuter, V E
AU  - Reuter VE
FAU - Bosl, G J
AU  - Bosl GJ
FAU - Chaganti, R S
AU  - Chaganti RS
LA  - eng
GR  - CA-05826/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Aneuploidy
MH  - Biomarkers, Tumor/*genetics
MH  - Biopsy
MH  - Centromere/ultrastructure
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 12/*ultrastructure
MH  - DNA Probes
MH  - DNA, Satellite
MH  - Female
MH  - Fluorescent Dyes
MH  - *Genetic Markers
MH  - Humans
MH  - Interphase
MH  - Male
MH  - Metaphase
MH  - Neoplasms, Germ Cell and Embryonal/*genetics/pathology
MH  - Nucleic Acid Hybridization
MH  - Polymorphism, Restriction Fragment Length
MH  - Recombination, Genetic
MH  - Tumor Cells, Cultured/ultrastructure
EDAT- 1991/07/01 00:00
MHDA- 1991/07/01 00:01
CRDT- 1991/07/01 00:00
PHST- 1991/07/01 00:00 [pubmed]
PHST- 1991/07/01 00:01 [medline]
PHST- 1991/07/01 00:00 [entrez]
AID - 10.1002/gcc.2870030409 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1991 Jul;3(4):300-7. doi: 10.1002/gcc.2870030409.