PMID- 16836076
OWN - NLM
STAT- MEDLINE
DCOM- 20110414
LR  - 20140625
VI  - 20
IP  - 9
DP  - 2006 May
TI  - [Methylation status of uPA promoter in laryngeal squamous cell carcinoma].
PG  - 409-11
AB  - OBJECTIVE: To investigate the relationship between methylation status of 
      urokinase type plasminogen activator (uPA) promoter and invasion/metastasis of 
      laryngeal squamous cell carcinoma (LSCC). METHOD: Using RT-PCR technique, 
      specimens from 40 patients of LSCC were detected for their expression of the uPA 
      gene. Meanwhile, methylation-specific PCR was used to detect methylation status 
      of uPA promoter. RESULT: Positive expression rate of uPAmRNA in LSCC was 65.0% 
      (26/40), and the rate of expression of uPAmRNA in LSCC with cervical lymph node 
      metastasis was higher than that of in LSCC without cervical lymph node metastasis 
      (P <0.05). Methylation of uPA promoter was observed in 8 (20.0%) of 40 cases of 
      LSCC, and the rate of methylation of uPA promoter in LSCC with cervical lymph 
      node metastasis was lower than that of in LSCC without cervical lymph node 
      metastasis (P <0.01). The expression of uPAmRNA was not detected in methylated 
      cancer tissues. CONCLUSION: Methylation leads to lose of the uPA gene expression 
      in LSCC. Demethylation of uPA promoter may be correlated to cervical lymph node 
      metastasis.
FAU - Wang, Dali
AU  - Wang D
AD  - Department of Otorhinolaryngology, the Second Affiliated Hospital, China Medical 
      University, Shenyang. wsln2000@sina.com
FAU - Wang, Tie
AU  - Wang T
FAU - Ren, Zhong
AU  - Ren Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Ke Za Zhi
JT  - Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology
JID - 9426080
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - *DNA Methylation
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - *Promoter Regions, Genetic
MH  - Urokinase-Type Plasminogen Activator/*metabolism
EDAT- 2006/07/14 09:00
MHDA- 2011/04/16 06:00
CRDT- 2006/07/14 09:00
PHST- 2006/07/14 09:00 [pubmed]
PHST- 2011/04/16 06:00 [medline]
PHST- 2006/07/14 09:00 [entrez]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 May;20(9):409-11.