PMID- 16836958
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20190917
IS  - 1031-3613 (Print)
IS  - 1031-3613 (Linking)
VI  - 18
IP  - 5
DP  - 2006
TI  - Effects of chronic prenatal ethanol exposure on mitochondrial glutathione and 
      8-iso-prostaglandin F2alpha concentrations in the hippocampus of the perinatal 
      guinea pig.
PG  - 517-24
AB  - It is hypothesised that oxidative stress is a key mechanism of ethanol 
      neurobehavioural teratogenicity, resulting in altered endogenous antioxidant 
      status and increased membrane lipid peroxidation in the hippocampus of chronic 
      prenatal ethanol exposure (CPEE) offspring. To test this hypothesis, timed 
      pregnant guinea-pigs (term, approximately gestational day (GD) 68) received 
      chronic daily oral administration of (i) 4 g ethanol kg(-1) maternal bodyweight, 
      (ii) isocaloric sucrose with pair feeding, or (iii) water. At GD 65 (term fetus) 
      and postnatal day (PD) 0 (neonate), individual offspring were killed, the brain 
      was excised and the hippocampi were dissected. Glutathione (GSH) concentration 
      was measured in the cytosolic and mitochondrial fractions of hippocampal 
      homogenate. The occurrence of lipid peroxidation was determined by measuring the 
      concentration of 8-iso-prostaglandin F2+/- (8-iso-PGF2+/-). There was 
      CPEE-induced decreased brain weight and hippocampal weight at GD 65 and PD 0, 
      decreased mitochondrial GSH concentration in the hippocampus at PD 0, with no 
      change in mitochondrial GSH concentration at GD 65 or cytosolic GSH concentration 
      at GD 65 or PD 0, and no change in mitochondrial or whole-homogenate 
      8-iso-PGF2+/- concentration in the hippocampus at GD 65 or PD 0. The data 
      demonstrate that CPEE produces selective mitochondrial dysfunction in the 
      hippocampus of the neonatal guinea-pig, involving GSH depletion.
FAU - Green, C R
AU  - Green CR
AD  - Department of Pharmacology and Toxicology, Queen's University, Kingston, ON K7L 
      3N6, Canada.
FAU - Watts, L T
AU  - Watts LT
FAU - Kobus, S M
AU  - Kobus SM
FAU - Henderson, G I
AU  - Henderson GI
FAU - Reynolds, J N
AU  - Reynolds JN
FAU - Brien, J F
AU  - Brien JF
LA  - eng
GR  - R0-5R21 AA013431/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 27415-26-5 (8-epi-prostaglandin F2alpha)
RN  - 3K9958V90M (Ethanol)
RN  - B7IN85G1HY (Dinoprost)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Birth Weight
MH  - Cytosol/chemistry
MH  - Dinoprost/*analogs & derivatives/analysis
MH  - Ethanol/*administration & dosage
MH  - Female
MH  - Fetal Weight
MH  - Gestational Age
MH  - Glutathione/*analysis
MH  - Guinea Pigs
MH  - Hippocampus/embryology/*ultrastructure
MH  - *Maternal-Fetal Exchange
MH  - Mitochondria/*chemistry
MH  - Organ Size
MH  - Pregnancy
EDAT- 2006/07/14 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/07/14 09:00
PHST- 2005/10/03 00:00 [received]
PHST- 2006/02/22 00:00 [accepted]
PHST- 2006/07/14 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/07/14 09:00 [entrez]
AID - RD05128 [pii]
AID - 10.1071/rd05128 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2006;18(5):517-24. doi: 10.1071/rd05128.