PMID- 16837193
OWN - NLM
STAT- MEDLINE
DCOM- 20130409
LR  - 20151119
IS  - 1879-0852 (Electronic)
IS  - 0959-8049 (Linking)
VI  - 49
IP  - 4
DP  - 2013 Mar
TI  - Tumor-associated antigenic pattern in squamous cell carcinomas of the head and 
      neck--analysed by SEREX.
PG  - e1-7
LID - S0959-8049(06)00216-4 [pii]
LID - 10.1016/j.ejca.2005.09.036 [doi]
AB  - Most immuno-therapeutic approaches are based on tumor-associated antigens and 
      many newly identified proteins have led to trials exploiting their possible 
      therapeutic applicability. So far only limited information on the antigenic 
      profile of head and neck squamous cell carcinomas (HNSCC) exists. Serological 
      analysis of tumor antigens by recombinant cDNA expression cloning (SEREX) was 
      used to identify the immunogenic patterns in our HNSCC patient collective. A cDNA 
      expression library derived from a pharynx HNSCC case was screened with autologous 
      and heterologous sera. Thirty-seven positive clones coding for 17 immunoreactive 
      gene products, which elicited an in vivo tumor response, were found. Results were 
      confirmed and extended by expression analysis using RT-PCR and in 
      situ-hybridisation. The protein-sequence of five clones exists so far only 
      hypothetically. Of all identified proteins only KIAA0530 has previously been 
      associated with a HNSCC related immune response. All other proteins have not yet 
      been described in a context with HNSCC antigenic patterns. Antibodies against a 
      heat shock transcription factor 2 (HSF2) were found in 2 out of 10 sera from 
      HNSCC patients. In summary, using the SEREX technique, we isolated 17 immunogenic 
      antigens in HNSCC's and confirmed the clinical relevance of KIAA0530. Further 
      analysis concerning their feasibility as target structures for an immunotherapy 
      approach is currently conducted.
CI  - Copyright (c) 2006. Published by Elsevier Ltd.
FAU - Heubeck, Bernd
AU  - Heubeck B
AD  - Department of Otorhinolaryngology Head and Neck Surgery, University of 
      Erlangen-Nuremberg, Waldstrasse 1, 91054 Erlangen, Germany. 
      uwe.mueller-vogt@hno.immed.uni-erlangen.de
FAU - Wendler, Olaf
AU  - Wendler O
FAU - Bumm, Klaus
AU  - Bumm K
FAU - Schafer, Renate
AU  - Schafer R
FAU - Muller-Vogt, Uwe
AU  - Muller-Vogt U
FAU - Hausler, Marcus
AU  - Hausler M
FAU - Meese, Eckart
AU  - Meese E
FAU - Iro, Heinrich
AU  - Iro H
FAU - Steinhart, Helmut
AU  - Steinhart H
LA  - eng
PT  - Journal Article
DEP - 20060711
PL  - England
TA  - Eur J Cancer
JT  - European journal of cancer (Oxford, England : 1990)
JID - 9005373
RN  - 0 (Antibodies, Neoplasm)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Antibodies, Neoplasm/*blood/genetics
MH  - Antigens, Neoplasm/*blood/genetics
MH  - Biomarkers, Tumor/*blood
MH  - Carcinoma, Squamous Cell/blood/genetics/*immunology
MH  - Gene Library
MH  - Head and Neck Neoplasms/blood/genetics/*immunology
MH  - Humans
MH  - In Situ Hybridization
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2006/07/14 09:00
MHDA- 2013/04/10 06:00
CRDT- 2006/07/14 09:00
PHST- 2005/07/21 00:00 [received]
PHST- 2005/09/06 00:00 [accepted]
PHST- 2006/07/14 09:00 [entrez]
PHST- 2006/07/14 09:00 [pubmed]
PHST- 2013/04/10 06:00 [medline]
AID - S0959-8049(06)00216-4 [pii]
AID - 10.1016/j.ejca.2005.09.036 [doi]
PST - ppublish
SO  - Eur J Cancer. 2013 Mar;49(4):e1-7. doi: 10.1016/j.ejca.2005.09.036. Epub 2006 Jul 
      11.