PMID- 16839409
OWN - NLM
STAT- MEDLINE
DCOM- 20070222
LR  - 20071115
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 36
IP  - 7
DP  - 2006 Jul
TI  - Surfactant protein D inhibits early airway response in Aspergillus 
      fumigatus-sensitized mice.
PG  - 930-40
AB  - BACKGROUND: The surfactant protein SP-D has been reported to reduce bronchial 
      hyper-responsiveness, blood eosinophilia, and T-helper type 2 cytokines in models 
      of allergic asthma. However, little is known about the functional effect of SP-D 
      on the early airway response upon allergen inhalation, which is an important 
      feature of this disease. OBJECTIVE: We investigated whether SP-D is able to 
      reduce the immediate allergen-induced mediator release and the early bronchial 
      obstruction in addition to its effects on airway inflammation and bronchial 
      hyperresponsiveness in an Aspergillus fumigatus mouse asthma model. METHODS: A. 
      fumigatus-sensitized mice were treated with a recombinant fragment of human SP-D 
      or placebo. Lung functions were measured in orotracheally intubated, 
      spontaneously breathing animals using body plethysmography. In addition, 
      passively sensitized precision-cut lung slices (PCLS) were used to determine the 
      effect of SP-D on allergen-induced histamine release. RESULTS: SP-D inhibited the 
      allergen-induced early airway response and reduced airway hyperresponsiveness 
      compared with placebo. Eosinophilia in bronchoalveolar lavage and lung tissue was 
      reduced after SP-D treatment, possibly by reducing eotaxin levels in the lung. 
      Furthermore, SP-D treatment reduced the allergen-induced histamine release from 
      PCLS. CONCLUSION: These data suggest that SP-D not only reduces allergen-induced 
      eosinophilic inflammation and airway hyperresponsiveness but also provides 
      protection against early airway obstruction by inhibition of early mediator 
      release.
FAU - Erpenbeck, V J
AU  - Erpenbeck VJ
AD  - Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany.
FAU - Ziegert, M
AU  - Ziegert M
FAU - Cavalet-Blanco, D
AU  - Cavalet-Blanco D
FAU - Martin, C
AU  - Martin C
FAU - Baelder, R
AU  - Baelder R
FAU - Glaab, T
AU  - Glaab T
FAU - Braun, A
AU  - Braun A
FAU - Steinhilber, W
AU  - Steinhilber W
FAU - Luettig, B
AU  - Luettig B
FAU - Uhlig, S
AU  - Uhlig S
FAU - Hoymann, H G
AU  - Hoymann HG
FAU - Krug, N
AU  - Krug N
FAU - Hohlfeld, J M
AU  - Hohlfeld JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Allergens)
RN  - 0 (Antigens, Fungal)
RN  - 0 (CCL11 protein, human)
RN  - 0 (Ccl11 protein, mouse)
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokines, CC)
RN  - 0 (Interleukin-5)
RN  - 0 (Pulmonary Surfactant-Associated Protein D)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Administration, Inhalation
MH  - Allergens/*immunology
MH  - Animals
MH  - Antigens, Fungal/immunology
MH  - Aspergillus fumigatus/*immunology
MH  - Asthma/immunology/metabolism/*prevention & control
MH  - Bronchial Hyperreactivity/immunology/metabolism/prevention & control
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Chemokine CCL11
MH  - Chemokines, CC/metabolism
MH  - Disease Models, Animal
MH  - Drug Evaluation, Preclinical
MH  - Eosinophilia/prevention & control
MH  - Female
MH  - Histamine Release/drug effects
MH  - Immunoglobulin E/blood
MH  - Interleukin-5/metabolism
MH  - Lung/metabolism
MH  - Lung Compliance
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Pulmonary Surfactant-Associated Protein D/pharmacokinetics/*therapeutic use
MH  - Recombinant Proteins/therapeutic use
EDAT- 2006/07/15 09:00
MHDA- 2007/02/23 09:00
CRDT- 2006/07/15 09:00
PHST- 2006/07/15 09:00 [pubmed]
PHST- 2007/02/23 09:00 [medline]
PHST- 2006/07/15 09:00 [entrez]
AID - CEA2524 [pii]
AID - 10.1111/j.1365-2222.2006.02524.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2006 Jul;36(7):930-40. doi: 10.1111/j.1365-2222.2006.02524.x.