PMID- 16839603
OWN - NLM
STAT- MEDLINE
DCOM- 20070928
LR  - 20181201
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 30
IP  - 12
DP  - 2006 Dec
TI  - Bone marrow derived mesenchymal stem cells from chronic myeloid leukemia t(9;22) 
      patients are devoid of Philadelphia chromosome and support cord blood stem cell 
      expansion.
PG  - 1493-8
AB  - Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder of 
      hematopoietic stem cells. It is characterized at cytogenetic level by the 
      Philadelphia (Ph) chromosome and at the molecular level by the BCR/ABL gene 
      rearrangement. Bone marrow derived mesenchymal stem cells (MSCs) are also 
      pluripotent stem cells that can differentiate into several mesenchymal tissues. 
      To date, no study has been performed to characterize whether MSCs from CML harbor 
      the abnormal Ph chromosome similar to CML bone marrow cells. We isolated and 
      characterized MSCs from diagnostic marrow samples (n=11) and showed that MSCs can 
      be readily isolated from CML marrow and exhibit major expansion potential as well 
      as intact osteogenic differentiation ability. Moreover, they do not harbor the Ph 
      chromosome confirmed by fluorescence in situ hybridization (FISH) and reverse 
      transcriptase polymerase chain reaction (RT-PCR). Thus, we demonstrated that CML 
      marrow is an abundant source of MSCs appearing through both FISH and RT-PCR not 
      to be involved by the malignant process of CML. Furthermore, these MSCs from a 
      CML patient could support in vitro cord blood expansion as those MSCs from a 
      normal donor. Since MSCs are able to support engraftment of hematopoietic stem 
      cells in stem cell transplantation (SCT) as well as suppress alloreactive T cells 
      causing graft-versus-host disease, this current report thus provides evidence 
      that in a SCT setting of CML patients, autologous MSCs could be a source of stem 
      cell support in future cell therapy applications.
FAU - Jootar, Saengsuree
AU  - Jootar S
AD  - Department of Internal Medicine, Faculty of Medicine, Ramathibodi Hospital, 
      Mahidol University, Bangkok, Thailand.
FAU - Pornprasertsud, Nida
AU  - Pornprasertsud N
FAU - Petvises, Sawang
AU  - Petvises S
FAU - Rerkamnuaychoke, Busaba
AU  - Rerkamnuaychoke B
FAU - Disthabanchong, Sinee
AU  - Disthabanchong S
FAU - Pakakasama, Samart
AU  - Pakakasama S
FAU - Ungkanont, Artit
AU  - Ungkanont A
FAU - Hongeng, Suradej
AU  - Hongeng S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060712
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Marrow Cells/*pathology
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Female
MH  - Fetal Blood/cytology
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis/*genetics
MH  - Male
MH  - Mesenchymal Stem Cells/*pathology
MH  - Middle Aged
MH  - *Philadelphia Chromosome
EDAT- 2006/07/15 09:00
MHDA- 2007/09/29 09:00
CRDT- 2006/07/15 09:00
PHST- 2006/01/24 00:00 [received]
PHST- 2006/04/04 00:00 [revised]
PHST- 2006/04/14 00:00 [accepted]
PHST- 2006/07/15 09:00 [pubmed]
PHST- 2007/09/29 09:00 [medline]
PHST- 2006/07/15 09:00 [entrez]
AID - S0145-2126(06)00149-4 [pii]
AID - 10.1016/j.leukres.2006.04.013 [doi]
PST - ppublish
SO  - Leuk Res. 2006 Dec;30(12):1493-8. doi: 10.1016/j.leukres.2006.04.013. Epub 2006 
      Jul 12.