PMID- 16839853
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20220331
IS  - 0741-8329 (Print)
IS  - 0741-8329 (Linking)
VI  - 38
IP  - 2
DP  - 2006 Feb
TI  - Increased expression of 5-HT1B receptors in rat nucleus accumbens via virally 
      mediated gene transfer increases voluntary alcohol consumption.
PG  - 73-9
AB  - Serotonin 5-HT(1B) receptors have been linked to alcoholism in humans and alcohol 
      consumption in rodents. We hypothesize that these receptors, which are located on 
      the axon terminals of nucleus accumbens' (NAcc) projection neurons, modulate 
      alcohol reward mechanisms. To test this hypothesis, we measured ethanol 
      consumption by rats that received bilateral microinjections of a viral vector 
      producing 5-HT(1B) overexpression (HA1B/GFP). Other groups received either 
      control (GFP-only) herpes simplex viral vectors into the medial NAcc shell or 
      were handled briefly with no surgery. All animals were housed singly and had 
      continuous access to water, 6% ethanol, and 12% ethanol in their home cages both 
      before and after surgery. There were no differences in the amount or rate of 
      weight gain, amount of food eaten, or total fluid consumed. There were also no 
      differences in the amount of ethanol consumed between groups prior to surgery. 
      However, after surgery, the HA1B/GFP group consumed twice as much ethanol as the 
      other groups. The main effect of total ethanol consumption was significant 
      (p<.05); the control groups did not differ from each other. Whereas there were no 
      between-group differences in 6% ethanol consumption, there was a large increase 
      in the amount of 12% ethanol consumed by the HA1B/GFP-expressing animals compared 
      to the two control groups as well as to their own presurgery intake (p<.05). We 
      hypothesize that increased 5-HT(1B) expression in NAcc led to either greater 
      reward or reduced aversive effects from the 12% ethanol, thereby leading to 
      increased voluntary ethanol consumption.
FAU - Hoplight, B J
AU  - Hoplight BJ
AD  - Department of Psychiatry and Behavioral Sciences, University of Washington 
      Medical School, Box 359911, Harborview Medical Center, 325 - 9th Avenue, Seattle, 
      WA 98104, USA.
FAU - Sandygren, N A
AU  - Sandygren NA
FAU - Neumaier, J F
AU  - Neumaier JF
LA  - eng
GR  - DA16432/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 0 (Hemagglutinins)
RN  - 0 (Receptor, Serotonin, 5-HT1B)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Alcohol Drinking/*physiopathology
MH  - Animals
MH  - Eating
MH  - Ethanol/administration & dosage
MH  - *Gene Expression
MH  - Genetic Vectors
MH  - Green Fluorescent Proteins/genetics
MH  - Hemagglutinins
MH  - Male
MH  - Nucleus Accumbens/*metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Receptor, Serotonin, 5-HT1B/*genetics/physiology
MH  - Recombinant Fusion Proteins
MH  - Simplexvirus/genetics
MH  - *Transfection
MH  - Weight Gain
EDAT- 2006/07/15 09:00
MHDA- 2006/11/15 09:00
CRDT- 2006/07/15 09:00
PHST- 2005/09/18 00:00 [received]
PHST- 2006/04/06 00:00 [revised]
PHST- 2006/04/11 00:00 [accepted]
PHST- 2006/07/15 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/07/15 09:00 [entrez]
AID - S0741-8329(06)00073-5 [pii]
AID - 10.1016/j.alcohol.2006.04.003 [doi]
PST - ppublish
SO  - Alcohol. 2006 Feb;38(2):73-9. doi: 10.1016/j.alcohol.2006.04.003.