PMID- 16840574
OWN - NLM
STAT- MEDLINE
DCOM- 20061128
LR  - 20130926
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 101
IP  - 5
DP  - 2006 Nov
TI  - Tumor necrosis factor-alpha promotes the accumulation of neutrophils and 
      macrophages in skeletal muscle.
PG  - 1394-9
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been associated with cachexia and is 
      known to regulate multiple inflammatory cell (neutrophil and macrophage) 
      responses. We tested the hypothesis that neutrophils and macrophages accumulate 
      in the extensor digitorum longus (EDL) and soleus muscles of mice after chronic 
      TNF-alpha administration. Murine recombinant TNF-alpha (approximately 100 microg 
      x kg(-1) x day(-1)) in vehicle solution or vehicle solution alone (sham) was 
      administered to C57BL/6 mice for 7 days via osmotic minipumps. In EDL muscles 
      from TNF-alpha-treated mice, neutrophil and macrophage concentrations were 
      elevated seven- and threefold, respectively, compared with sham mice. Neutrophil 
      and macrophage concentrations were also elevated five- and twofold, respectively, 
      in solei of TNF-alpha- relative to sham-treated mice. Treatment with TNF-alpha 
      elevated ubiquitin content by approximately 25% relative to sham values for both 
      the EDL and soleus muscles; however, these elevations were not statistically 
      significant. No differences were observed between TNF-alpha- and sham-treated 
      mice in body weight, food consumption, muscle mass, myofiber cross-sectional 
      area, carbonyl groups, total protein content, or relative abundance of myosin 
      heavy chain protein. Furthermore, no overt signs of muscle injury or regeneration 
      were observed in muscles from TNF-alpha-treated mice in either the EDL or soleus 
      muscles. These observations suggest that 7 days of TNF-alpha administration 
      promote muscle inflammation as indicated by the accumulation of neutrophils and 
      macrophages without overt signs of atrophy, injury, or regeneration.
FAU - Peterson, Jennifer M
AU  - Peterson JM
AD  - Department of Kinesiology, The University of Toledo, Toledo, OH 43606, USA.
FAU - Feeback, Kevin D
AU  - Feeback KD
FAU - Baas, Joel H
AU  - Baas JH
FAU - Pizza, Francis X
AU  - Pizza FX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060713
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Ubiquitin)
SB  - IM
MH  - Animals
MH  - Infusion Pumps, Implantable
MH  - Macrophages/*drug effects/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/*drug effects/pathology
MH  - Myositis/*chemically induced/pathology
MH  - Neutrophils/*drug effects/pathology
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Ubiquitin/metabolism
EDAT- 2006/07/15 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/07/15 09:00
PHST- 2006/07/15 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/07/15 09:00 [entrez]
AID - 01453.2005 [pii]
AID - 10.1152/japplphysiol.01453.2005 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2006 Nov;101(5):1394-9. doi: 
      10.1152/japplphysiol.01453.2005. Epub 2006 Jul 13.