PMID- 16841034
OWN - NLM
STAT- MEDLINE
DCOM- 20061214
LR  - 20220311
IS  - 1523-1747 (Electronic)
IS  - 0022-202X (Linking)
VI  - 126
IP  - 12
DP  - 2006 Dec
TI  - Green tea extract and (-)-epigallocatechin-3-gallate inhibit mast cell-stimulated 
      type I collagen expression in keloid fibroblasts via blocking PI-3K/AkT signaling 
      pathways.
PG  - 2607-13
AB  - Keloid, a chronic fibro-proliferative disease, exhibits distinctive histological 
      features characterized by an abundant extracellular matrix stroma, a local 
      infiltration of inflammatory cells including mast cells (MCs), and a milieu of 
      enriched cytokines. Previous studies have demonstrated that co-culture with MCs 
      stimulate type I collagen synthesis in fibroblasts, but the signaling mechanisms 
      remain largely unknown. In this study, we investigated the signaling pathways 
      involved in MC-stimulated type I collagen synthesis and the effects of green tea 
      extract (GTE) and its major catechin, (-)-epigallocatechin-3-gallate (EGCG), on 
      collagen homeostasis in keloid fibroblasts. Our results showed that MCs 
      significantly stimulated type I collagen expression in keloid fibroblasts, and 
      the upregulation of type I collagen was significantly attenuated by blockade of 
      phosphatidylinositol-3-kinase (PI-3K), mammalian target of rapamycin (mTOR), and 
      p38 MAPK signaling pathways, but not by blockade of ERK1/2 pathway. Furthermore, 
      GTE and EGCG dramatically inhibited type I collagen production possibly by 
      interfering with the PI-3K/Akt/mTOR signaling pathway. Our findings suggest that 
      interaction between MCs and keloid fibroblasts may contribute to excessive 
      collagen accumulation in keloids and imply a therapeutic potential of green tea 
      for the intervention and prevention of keloids and other fibrotic diseases.
FAU - Zhang, Qunzhou
AU  - Zhang Q
AD  - Center for Craniofacial Molecular Biology, University of Southern California, 
      School of Dentistry, Los Angeles, California 90033, USA.
FAU - Kelly, A Paul
AU  - Kelly AP
FAU - Wang, Lina
AU  - Wang L
FAU - French, Samuel W
AU  - French SW
FAU - Tang, Xudong
AU  - Tang X
FAU - Duong, Hai S
AU  - Duong HS
FAU - Messadi, Diana V
AU  - Messadi DV
FAU - Le, Anh D
AU  - Le AD
LA  - eng
GR  - S11 AR47359/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060713
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Collagen Type I)
RN  - 0 (Plant Extracts)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Camellia sinensis/*chemistry
MH  - Catechin/*analogs & derivatives/pharmacology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Collagen Type I/*metabolism
MH  - Fibroblasts/*metabolism
MH  - Humans
MH  - Keloid/*metabolism/pathology
MH  - Mast Cells/*physiology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Plant Extracts/*pharmacology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction/drug effects
EDAT- 2006/07/15 09:00
MHDA- 2006/12/15 09:00
CRDT- 2006/07/15 09:00
PHST- 2006/07/15 09:00 [pubmed]
PHST- 2006/12/15 09:00 [medline]
PHST- 2006/07/15 09:00 [entrez]
AID - S0022-202X(15)32708-1 [pii]
AID - 10.1038/sj.jid.5700472 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2006 Dec;126(12):2607-13. doi: 10.1038/sj.jid.5700472. Epub 
      2006 Jul 13.