PMID- 16842741
OWN - NLM
STAT- MEDLINE
DCOM- 20060913
LR  - 20091119
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 347
IP  - 3
DP  - 2006 Sep 1
TI  - CART peptide promotes the survival of hippocampal neurons by upregulating 
      brain-derived neurotrophic factor.
PG  - 656-61
AB  - The neuropeptide cocaine- and amphetamine-regulated transcript (CARTp) plays a 
      role in various physiological processes. CARTp is highly expressed in rat 
      hippocampus and can promote the survival and differentiation of neurons in 
      primary hippocampal cell cultures. However, little is known about the 
      neurotrophic mechanism of CARTp on the hippocampal neuron. We show that CARTp 
      fragment 55-102 promoted the survival of cultured hippocampal neurons by 
      increasing the number of surviving neurons and their viability. The tyrosine 
      kinase B (TrkB) antibody, known to inhibit the activity of brain-derived 
      neurotrophic factor (BDNF), blocked the survival-promoting effect of CARTp on 
      hippocampal neurons. Further study by reverse-transcription PCR showed that BDNF 
      mRNA expression significantly increased after CARTp treatment. The prepro BDNF 
      and mature BDNF protein also increased in level as seen on Western blot analysis. 
      Thus, the neurotrophic effects of CARTp on cultured hippocampal neurons are 
      mediated through the upregulation of BDNF mRNA expression and protein synthesis. 
      The results of the present study suggest the therapeutic efficacy of CARTp in 
      neurodegenerative disorders.
FAU - Wu, Bin
AU  - Wu B
AD  - Department of Physiology and Pathophysiology, Peking University Health Science 
      Center, Beijing, People's Republic of China.
FAU - Hu, Shengdi
AU  - Hu S
FAU - Yang, Min
AU  - Yang M
FAU - Pan, Hui
AU  - Pan H
FAU - Zhu, Shigong
AU  - Zhu S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060628
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Hippocampus/cytology/*drug effects/*metabolism
MH  - Nerve Growth Factor/genetics
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Peptide Fragments/*pharmacology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/metabolism
MH  - Up-Regulation/*drug effects
EDAT- 2006/07/18 09:00
MHDA- 2006/09/14 09:00
CRDT- 2006/07/18 09:00
PHST- 2006/06/17 00:00 [received]
PHST- 2006/06/21 00:00 [accepted]
PHST- 2006/07/18 09:00 [pubmed]
PHST- 2006/09/14 09:00 [medline]
PHST- 2006/07/18 09:00 [entrez]
AID - S0006-291X(06)01430-6 [pii]
AID - 10.1016/j.bbrc.2006.06.117 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Sep 1;347(3):656-61. doi: 
      10.1016/j.bbrc.2006.06.117. Epub 2006 Jun 28.