PMID- 16842902
OWN - NLM
STAT- MEDLINE
DCOM- 20070806
LR  - 20121115
IS  - 0301-4797 (Print)
IS  - 0301-4797 (Linking)
VI  - 83
IP  - 4
DP  - 2007 Jun
TI  - Effect of feeding time on the performance of a sequencing batch reactor treating 
      a mixture of 4-CP and 2,4-DCP.
PG  - 427-36
AB  - This paper investigated the biodegradation kinetics of 4-chlorophenol (4-CP) and 
      2,4-dichlorophenol (2,4-DCP) separately in batch reactors and mixed in sequencing 
      batch reactors (SBRs). Batch reactor experiments showed that both 4-CP and 
      2,4-DCP began to inhibit their own degradation at 53 and 25 mg l(-1), 
      respectively, and that the Haldane equation gave a good fit to the experimental 
      data because r(2) values were higher than 0.98. The maximum specific degradation 
      rates (q(m)) were 130.3 and 112.4 mg g(-1) h for 4-CP and 2,4-DCP, respectively. 
      The values of the half saturation (K(s)) and self-inhibition constants (K(i)) 
      were 34.98 and 79.74 mg l(-1) for 4-CP, and 13.77 and 44.46 mg l(-1) for 2,4-DCP, 
      respectively. The SBR was fed with a mixture of 220 mg l(-1) of 4-CP, 110 mg 
      l(-1) of 2,4-DCP, and 300 mg l(-1) of peptone as biogenic substrate at varying 
      feeding periods (0-8h) to evaluate the effect of feeding time on the performance 
      of the SBR. During SBR operation, in addition to self-inhibition, 4-CP 
      degradation was strongly and competitively inhibited by 2,4-DCP. The inhibitory 
      effects were particularly pronounced during short feeding periods because of 
      higher chlorophenol peak concentrations in the reactor. The competitive 
      inhibition constant (K(ii)) of 2,4-DCP on 4-CP degradation was 0.17 mg l(-1) when 
      the reactor was fed instantaneously (0 h feeding). During longer feedings, 
      increased removal/loading rates led to lower chlorophenol peak concentrations at 
      the end of feeding. Therefore, in multi-substrate systems feeding time plus 
      reaction time should be determined based on both degradation kinetics and 
      substrate interaction. During degradation, the meta cleavage of 4-chlorocatechol 
      resulted in accumulation of a yellowish color because of the formation of 
      5-chloro-2-hydroxymuconic semialdehyde (CHMS), which was further metabolized. 
      Isolation and enrichment of the chlorophenols-degrading culture suggested 
      Pseudomonas sp. and Pseudomonas stutzeri to be the dominant species.
FAU - Sahinkaya, Erkan
AU  - Sahinkaya E
AD  - Middle East Technical University, Environmental Engineering Department, 06531 
      Ankara, Turkey. erkans@metu.edu.tr
FAU - Dilek, Filiz B
AU  - Dilek FB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060713
PL  - England
TA  - J Environ Manage
JT  - Journal of environmental management
JID - 0401664
RN  - 0 (Chlorophenols)
RN  - 0 (Water Pollutants, Chemical)
RN  - 3DLC36A01X (4-chlorophenol)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Bacteria/metabolism
MH  - *Bioreactors
MH  - Chlorophenols/*metabolism
MH  - Kinetics
MH  - Time Factors
MH  - Waste Disposal, Fluid
MH  - Water Pollutants, Chemical/*metabolism
EDAT- 2006/07/18 09:00
MHDA- 2007/08/07 09:00
CRDT- 2006/07/18 09:00
PHST- 2005/05/19 00:00 [received]
PHST- 2006/02/07 00:00 [revised]
PHST- 2006/04/01 00:00 [accepted]
PHST- 2006/07/18 09:00 [pubmed]
PHST- 2007/08/07 09:00 [medline]
PHST- 2006/07/18 09:00 [entrez]
AID - S0301-4797(06)00137-X [pii]
AID - 10.1016/j.jenvman.2006.04.007 [doi]
PST - ppublish
SO  - J Environ Manage. 2007 Jun;83(4):427-36. doi: 10.1016/j.jenvman.2006.04.007. Epub 
      2006 Jul 13.