PMID- 16843011
OWN - NLM
STAT- MEDLINE
DCOM- 20071106
LR  - 20081121
IS  - 1050-4648 (Print)
IS  - 1050-4648 (Linking)
VI  - 22
IP  - 3
DP  - 2007 Mar
TI  - Early local and systemic innate immune responses in the teleost gilthead seabream 
      after intraperitoneal injection of whole yeast cells.
PG  - 242-51
AB  - The early cellular innate immune responses of the teleost gilthead seabream 
      (Sparus aurata L.) against whole yeast cells were studied. Fish received a single 
      intraperitoneal (i.p.) injection of Saccharomyces cerevisiae and leukocyte 
      mobilization, degranulation, peroxidase content, respiratory burst, phagocytic 
      and cytotoxic activities were assayed in both head-kidney leukocytes (HKLs) and 
      peritoneal exudate leukocytes (PELs). The total number of PELs significantly 
      increased from 4 h post-injection until the end of the experiment (3 days). 
      Interestingly, flow cytometric analysis revealed variations in the proportion of 
      cell-types in the PE. Thus, PE acidophilic granulocytes increased to a 
      significant extent 4 h post-injection and were restored thereafter. Moreover, PE 
      monocyte-macrophages started to increase from 24 h, the enhancement being 
      statistically significant after 48 and 72 h. Degranulation was greater in PELs 
      throughout the assay. The peroxidase content of the leukocytes was affected 
      differently in HKLs and PELs. The respiratory burst activity was not affected in 
      HKLs but significantly increased in PELs from 4 to 48 h post-injection with yeast 
      cells. On the other hand, HKL phagocytosis had decreased 72 h post-injection with 
      yeast cells while it increased after 4 and 24 h post-injection in the PELs. 
      Conversely, the cytotoxic activity was significantly enhanced in HKLs from 24 to 
      72 h post-injection but slightly decreased in PELs. Finally, our data demonstrate 
      that seabream injected with the yeast Saccharomyces cerevisiae show leukocyte 
      mobilization and cellular innate immune response activation at the site of 
      invasion and also in the head-kidney. The implications of the leukocyte-types and 
      the immune responses observed, as well as analogies with other particulated 
      antigens, will be discussed as possible models for investigating the effect of 
      potential pathogens.
FAU - Cuesta, Alberto
AU  - Cuesta A
AD  - Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, 
      University of Murcia, 30100 Murcia, Spain.
FAU - Rodriguez, Alejandro
AU  - Rodriguez A
FAU - Salinas, Irene
AU  - Salinas I
FAU - Meseguer, Jose
AU  - Meseguer J
FAU - Esteban, M Angeles
AU  - Esteban MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060527
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Cell Degranulation/immunology
MH  - Cytotoxicity, Immunologic
MH  - Flow Cytometry/veterinary
MH  - Immunity, Cellular
MH  - Immunity, Innate/*immunology
MH  - Injections, Intraperitoneal/veterinary
MH  - Leukocytes/enzymology/*immunology/physiology
MH  - Peroxidase/analysis
MH  - Phagocytosis/immunology
MH  - Random Allocation
MH  - Respiratory Burst/immunology
MH  - Saccharomyces cerevisiae/*immunology
MH  - Sea Bream/*immunology
MH  - Time Factors
EDAT- 2006/07/18 09:00
MHDA- 2007/11/07 09:00
CRDT- 2006/07/18 09:00
PHST- 2006/03/29 00:00 [received]
PHST- 2006/05/17 00:00 [revised]
PHST- 2006/05/22 00:00 [accepted]
PHST- 2006/07/18 09:00 [pubmed]
PHST- 2007/11/07 09:00 [medline]
PHST- 2006/07/18 09:00 [entrez]
AID - S1050-4648(06)00089-1 [pii]
AID - 10.1016/j.fsi.2006.05.005 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2007 Mar;22(3):242-51. doi: 10.1016/j.fsi.2006.05.005. 
      Epub 2006 May 27.