PMID- 16843500
OWN - NLM
STAT- MEDLINE
DCOM- 20061103
LR  - 20061115
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 79
IP  - 19
DP  - 2006 Oct 4
TI  - Bacterial endotoxin lipopolysaccharide induces up-regulation of 
      glyceraldehyde-3-phosphate dehydrogenase in rat liver and lungs.
PG  - 1820-7
AB  - Bacterial endotoxin or lipopolysaccharide (LPS) can trigger inflammatory 
      responses and cause damage in organs such as liver and lungs when it is 
      introduced into mammals, but the exact molecular events that mediate these 
      responses have remained obscure. In this study, by using 2D gel electrophoresis 
      and cDNA microarray analysis, we found that both protein and mRNA levels of 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were significantly increased in 
      rat liver and lungs after treatment with LPS. The results were further confirmed 
      by Western blot and Northern blot. Given the known role of GAPDH in inducing 
      apoptosis, our results suggest that LPS-induced GAPDH up-regulation may be an 
      important mechanism responsible for the damage induced by Gram negative bacteria 
      in mammalian tissue and GAPDH may be involved in the signaling pathway of LPS 
      induced apoptosis. Our results also demonstrate that GAPDH is not a suitable 
      internal control in gene expression studies, especially when bacterial infection 
      is involved.
FAU - Xie, Wenguang
AU  - Xie W
AD  - Affiliated Hospital, North Sichuan Medical College, Nanchong, China. 
      xiewenguangcb@yahoo.com
FAU - Shao, Ningsheng
AU  - Shao N
FAU - Ma, Xiaochang
AU  - Ma X
FAU - Ling, Baodong
AU  - Ling B
FAU - Wei, Yushu
AU  - Wei Y
FAU - Ding, Qinxue
AU  - Ding Q
FAU - Yang, Guang
AU  - Yang G
FAU - Liu, Nongle
AU  - Liu N
FAU - Wang, Huixin
AU  - Wang H
FAU - Chen, Keji
AU  - Chen K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060618
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Enzyme Induction/drug effects
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*biosynthesis/genetics
MH  - Injections, Intravenous
MH  - Lipopolysaccharides/*toxicity
MH  - Liver/*drug effects/enzymology/pathology
MH  - Lung/*drug effects/enzymology/pathology
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Up-Regulation
EDAT- 2006/07/18 09:00
MHDA- 2006/11/04 09:00
CRDT- 2006/07/18 09:00
PHST- 2005/12/20 00:00 [received]
PHST- 2006/05/16 00:00 [revised]
PHST- 2006/06/10 00:00 [accepted]
PHST- 2006/07/18 09:00 [pubmed]
PHST- 2006/11/04 09:00 [medline]
PHST- 2006/07/18 09:00 [entrez]
AID - S0024-3205(06)00460-7 [pii]
AID - 10.1016/j.lfs.2006.06.018 [doi]
PST - ppublish
SO  - Life Sci. 2006 Oct 4;79(19):1820-7. doi: 10.1016/j.lfs.2006.06.018. Epub 2006 Jun 
      18.