PMID- 16844231
OWN - NLM
STAT- MEDLINE
DCOM- 20061031
LR  - 20231213
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 106
IP  - 2
DP  - 2006 Aug 15
TI  - Enhancement of dendritic cell-based vaccine potency by targeting antigen to 
      endosomal/lysosomal compartments.
PG  - 126-34
AB  - Dendritic cells (DCs) are the central players in cancer immunotherapy because of 
      their distinct ability to prime immune responses. In previous work with DNA 
      vaccines, we described an intracellular targeting approach that routed a 
      nuclear/cytoplasmic antigen, human papillomavirus (HPV) type 16 E7, into the 
      endosomal and lysosomal compartments. It does so by linking E7 with the sorting 
      signal of lysosome-associated membrane protein 1 (Sig/LAMP-1) to enhance the 
      presentation of E7 antigen to MHC class I-restricted CD8(+) T cells, as well as 
      to MHC class II-restricted CD4(+) T cells. To date, the Sig/LAMP-1 targeting 
      strategy has not been tested in the context of DC-based vaccines. This study was 
      designed to determine whether targeting HPV-16 E7 to the endosomal/lysosomal 
      compartment can enhance the potency of DC vaccines. In immunological studies, 
      DC-Sig/E7/LAMP-1 dramatically increased in vitro activation and in vivo expansion 
      of E7-specific CD4(+) and CD8(+) T cells, compared with DC-E7 and DC-No insert. 
      More importantly, in both tumor prevention and tumor treatment assays, 
      DC-Sig/E7/LAMP-1 generated greater anti-tumor immunity against TC-1 than DC-E7. 
      Our results demonstrate that linkage of the antigen gene to an 
      endosomal/lysosomal targeting signal may greatly enhance the potency of DC-based 
      vaccines.
FAU - Kang, Tae Heung
AU  - Kang TH
AD  - Laboratory of Infection and Immunology, Graduate School of Medicine, Korea 
      University, 516 Gojan-1 Dong, Ansan-Si, Gyeonggi-Do 425-707, South Korea.
FAU - Lee, Jin Hyup
AU  - Lee JH
FAU - Bae, Hyun Cheol
AU  - Bae HC
FAU - Noh, Kyung Hee
AU  - Noh KH
FAU - Kim, Jin Hee
AU  - Kim JH
FAU - Song, Chung Kil
AU  - Song CK
FAU - Shin, Byung Chul
AU  - Shin BC
FAU - Hung, Chien-Fu
AU  - Hung CF
FAU - Wu, T-C
AU  - Wu TC
FAU - Park, Jong-Sup
AU  - Park JS
FAU - Kim, Tae Woo
AU  - Kim TW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060522
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Cancer Vaccines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lamp1 protein, mouse)
RN  - 0 (Lysosomal Membrane Proteins)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Papillomavirus E7 Proteins)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (oncogene protein E7, Human papillomavirus type 16)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/genetics/immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cancer Vaccines/genetics/*immunology
MH  - Dendritic Cells/*immunology/transplantation
MH  - Endosomes/genetics/*immunology
MH  - Female
MH  - Histocompatibility Antigens Class II/immunology
MH  - Lymphocyte Activation/genetics/immunology
MH  - Lysosomal Membrane Proteins/genetics/immunology
MH  - Lysosomes/genetics/*immunology
MH  - Mice
MH  - Neoplasms, Experimental/genetics/*immunology
MH  - Oncogene Proteins, Viral/genetics/*immunology
MH  - Papillomavirus E7 Proteins
MH  - Protein Sorting Signals/genetics
EDAT- 2006/07/18 09:00
MHDA- 2006/11/01 09:00
CRDT- 2006/07/18 09:00
PHST- 2005/12/13 00:00 [received]
PHST- 2006/04/17 00:00 [revised]
PHST- 2006/05/01 00:00 [accepted]
PHST- 2006/07/18 09:00 [pubmed]
PHST- 2006/11/01 09:00 [medline]
PHST- 2006/07/18 09:00 [entrez]
AID - S0165-2478(06)00130-1 [pii]
AID - 10.1016/j.imlet.2006.05.004 [doi]
PST - ppublish
SO  - Immunol Lett. 2006 Aug 15;106(2):126-34. doi: 10.1016/j.imlet.2006.05.004. Epub 
      2006 May 22.