PMID- 16845520
OWN - NLM
STAT- MEDLINE
DCOM- 20061121
LR  - 20181113
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 113
IP  - 5
DP  - 2006 Sep
TI  - Identification and fine mapping of AvrPi15, a novel avirulence gene of 
      Magnaporthe grisea.
PG  - 875-83
AB  - Avirulence of Magnaporthe grisea isolate CHL346 on rice cultivar GA25 was studied 
      with 242 ascospore progenies derived from the cross CHL346 x CHL42. Segregation 
      analysis of the avirulence in the progeny population was in agreement with the 
      existence of a single avirulence (Avr) gene, designated as AvrPi15. For mapping 
      the Avr gene, we developed a total of 121 microsatellite DNA markers [simple 
      sequence repeat (SSR)], which evenly distributed in the whole-genome of M. grisea 
      through bioinformatics analysis (BIA) using the publicly available sequence. 
      Linkage analysis of the AvrPi15 gene with these SSR markers showed that six 
      markers on chromosome 6, MS6-1, MS6-2, MS6-3, MS6-7, MS6-8 and MS6-10, were 
      linked to the AvrPi15 locus. To further define the chromosomal location of the 
      AvrPi15 locus, two additional markers, MS6-17 and STS6-6, which were developed 
      based on the sequences of telomeric region 11 (TEL11), were subjected to linkage 
      analysis. The results showed that MS6-17 and STS6-6 were associated with the 
      locus by 3.3 and 0.8 cM, respectively. To finely map the Avr gene, two additional 
      candidate avirulence gene (CAG) markers, CAG6-1 and CAG6-2, were developed based 
      on the gene annotation of the sequence of TEL 11. Linkage analysis of the Avr 
      gene with these two markers revealed that both of them completely cosegregated 
      with the AvrPi15 locus. Finally, this locus was physically mapped into 
      approximately 7.2-kb interval of the TEL11 by BIA using these sequence-ready 
      markers. This is the key step toward positional cloning of the AvrPi15 gene.
FAU - Ma, Jun-Hong
AU  - Ma JH
AD  - Laboratory of Plant Resistance and Genetics, College of Resources and 
      Environmental Sciences, South China Agricultural University, Guangzhou 510642, 
      China.
FAU - Wang, Ling
AU  - Wang L
FAU - Feng, Shu-Jie
AU  - Feng SJ
FAU - Lin, Fei
AU  - Lin F
FAU - Xiao, Yi
AU  - Xiao Y
FAU - Pan, Qing-Hua
AU  - Pan QH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060715
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Segregation
MH  - Chromosomes, Plant
MH  - Computational Biology
MH  - Crosses, Genetic
MH  - DNA Primers
MH  - Genes, Plant/*genetics
MH  - Genetic Markers
MH  - Genome, Plant
MH  - Immunity, Innate/*genetics
MH  - Magnaporthe/*genetics
MH  - Microsatellite Repeats
MH  - *Physical Chromosome Mapping
MH  - Plant Diseases/genetics/*microbiology
EDAT- 2006/07/18 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/07/18 09:00
PHST- 2006/01/04 00:00 [received]
PHST- 2006/06/13 00:00 [accepted]
PHST- 2006/07/18 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/07/18 09:00 [entrez]
AID - 10.1007/s00122-006-0347-6 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2006 Sep;113(5):875-83. doi: 10.1007/s00122-006-0347-6. Epub 
      2006 Jul 15.