PMID- 16845607
OWN - NLM
STAT- MEDLINE
DCOM- 20060929
LR  - 20161124
IS  - 1420-682X (Print)
IS  - 1420-682X (Linking)
VI  - 63
IP  - 15
DP  - 2006 Aug
TI  - Translation control of UCP2 synthesis by the upstream open reading frame.
PG  - 1780-9
AB  - Uncoupling protein 2 (UCP2) belongs to a family of transporters of the 
      mitochondrial inner membrane. In vivo low expression of UCP2 contrasts with a 
      high UCP2 mRNA level, and induction of UCP2 expression occurs without change in 
      mRNA level, demonstrating a translational control. The UCP2 mRNA is characterized 
      by a long 5' untranslated region (5'UTR), in which an upstream open reading frame 
      (uORF) codes for a 36-amino-acid sequence. The 5'UTR and uORF have an inhibitory 
      role in the translation of UCP2. The present study demonstrates that the 3' 
      region of the uORF is a major determinant for this inhibitory role. In this 3' 
      region, a single-base substitution that kept the codon sense unchanged 
      significantly modified UCP2 translation, whereas some important amino acid 
      changes had no effect. We discuss our results within the framework of the 
      existing models explaining initiation of translation downstream of a uORF.
FAU - Hurtaud, C
AU  - Hurtaud C
AD  - BIOTRAM, CNRS UPR9078, Faculte de Medecine Necker Enfants Malades, Universite 
      Rene Descartes, 75730 Paris, France.
FAU - Gelly, C
AU  - Gelly C
FAU - Bouillaud, F
AU  - Bouillaud F
FAU - Levi-Meyrueis, C
AU  - Levi-Meyrueis C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cell Mol Life Sci
JT  - Cellular and molecular life sciences : CMLS
JID - 9705402
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Ion Channels)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Uncoupling Protein 2)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Ion Channels
MH  - Membrane Transport Proteins/*biosynthesis/genetics
MH  - Mitochondrial Proteins/*biosynthesis/genetics
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - *Open Reading Frames
MH  - RNA, Messenger/*biosynthesis
MH  - Transcription, Genetic
MH  - Uncoupling Protein 2
EDAT- 2006/07/18 09:00
MHDA- 2006/09/30 09:00
CRDT- 2006/07/18 09:00
PHST- 2006/07/18 09:00 [pubmed]
PHST- 2006/09/30 09:00 [medline]
PHST- 2006/07/18 09:00 [entrez]
AID - 10.1007/s00018-006-6129-0 [doi]
PST - ppublish
SO  - Cell Mol Life Sci. 2006 Aug;63(15):1780-9. doi: 10.1007/s00018-006-6129-0.