PMID- 16847329
OWN - NLM
STAT- MEDLINE
DCOM- 20060919
LR  - 20181113
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 26
IP  - 15
DP  - 2006 Aug
TI  - Mechanism of action of a distal NF-kappaB-dependent enhancer.
PG  - 5759-70
AB  - The monocyte chemoattractant protein 1 gene (MCP-1) is regulated by TNF through 
      an NF-kappaB-dependent distal enhancer and an Sp1-dependent promoter-proximal 
      regulatory region. In the silent state, only the distal regulatory region is 
      accessible to transcription factors. Upon activation by tumor necrosis factor, 
      NF-kappaB binds to the distal regulatory region and recruits CBP and p300. CBP 
      and p300 recruitment led to specific histone modifications that ultimately 
      enabled the binding of Sp1 to the proximal regulatory region. During this 
      process, a direct interaction between the distal and proximal regulatory regions 
      occurred. Sp1, NF-kappaB, CBP, and p300 were required for this interaction. 
      CBP/p300-mediated histone modifications enhanced the binding of the coactivator 
      CARM1 to the distal regulatory region. CARM1, which is necessary for MCP-1 
      expression, was not required for distal-proximal region interactions, suggesting 
      that it plays a later downstream activation event. The results describe a model 
      in which the separation of the distal enhancer from the promoter-proximal region 
      allows for two independent chromatin states to exist, preventing inappropriate 
      gene activation at the promoter while at the same time allowing rapid induction 
      through the distal regulatory region.
FAU - Teferedegne, Belete
AU  - Teferedegne B
AD  - Department of Microbiology and Immunology, Emory University School of Medicine, 
      Atlanta, GA 30322, USA.
FAU - Green, Myesha R
AU  - Green MR
FAU - Guo, Zhu
AU  - Guo Z
FAU - Boss, Jeremy M
AU  - Boss JM
LA  - eng
GR  - R01 CA096810/CA/NCI NIH HHS/United States
GR  - CA96810/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chromatin)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.1.1.319 (Protein-Arginine N-Methyltransferases)
RN  - EC 2.1.1.319 (coactivator-associated arginine methyltransferase 1)
RN  - EC 2.3.1.48 (p300-CBP Transcription Factors)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Chromatin/chemistry/metabolism
MH  - *Enhancer Elements, Genetic
MH  - Gene Expression Regulation
MH  - Genes, Reporter
MH  - Mice
MH  - NF-kappa B/*genetics/metabolism
MH  - NIH 3T3 Cells
MH  - Protein Structure, Tertiary
MH  - Protein-Arginine N-Methyltransferases/genetics/metabolism
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Sp1 Transcription Factor/genetics/metabolism
MH  - Transcription Factor RelA/genetics/metabolism
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - p300-CBP Transcription Factors/genetics/metabolism
PMC - PMC1592769
EDAT- 2006/07/19 09:00
MHDA- 2006/09/20 09:00
PMCR- 2007/02/01
CRDT- 2006/07/19 09:00
PHST- 2006/07/19 09:00 [pubmed]
PHST- 2006/09/20 09:00 [medline]
PHST- 2006/07/19 09:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - 26/15/5759 [pii]
AID - 0271-06 [pii]
AID - 10.1128/MCB.00271-06 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2006 Aug;26(15):5759-70. doi: 10.1128/MCB.00271-06.