PMID- 16847529
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20061115
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 8
IP  - 5
DP  - 2006 Sep
TI  - Immunohistopathology of the contralateral testis of rats undergoing experimental 
      torsion of the spermatic cord.
PG  - 576-83
AB  - AIM: To evaluate the immunohistopathological changes in the contralateral testis 
      of rats after an experimental spermatic cord torsion. METHODS: Male 
      Sprague-Dawley rats of 45-50 days old were subjected to a 720 degree unilateral 
      spermatic cord torsion for 10, 30 and 80 days (experimental group, E), 
      respectively or sham operation (control group, C). Histopathology of the 
      contralateral testis as well as germ cell apoptosis were studied using the 
      Terminal Deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL) 
      technique. The number of testicular lymphocytes, mast cells and macrophages, and 
      the expression of tumor necrosis factor-alpha (TNF-alpha) and its receptor 
      (TNFR1) in testicular cells of the contralateral testis were quantified by 
      histochemistry and immunohistochemistry. TNF-alpha concentration in testicular 
      fluid was determined by ELISA. RESULTS: In the contralateral testis of rats from 
      the E group, the maximal degree of damage of the germinal epithelium was seen 30 
      days after torsion. At this time we observed in the E group vs. the C group 
      increases: (i) the number of testicular T-lymphocytes; (ii) the number of 
      testicular mast cells and macrophages; (iii) the percentage of macrophages 
      expressing TNF-alpha; (iv) TNF-a concentration in testicular fluid; (v) the 
      number of apoptotic germ cells; and (vi) the number of TNFR1+ germ cells. 
      CONCLUSION: Experimental spermatic cord torsion induces, in the contralateral 
      testis, a focal damage of seminiferous tubules characterized by apoptosis and 
      sloughing of germ cells. Results suggest humoral and cellular immune mediated 
      testicular cell damage in which macrophages and mast cells seem to be involved in 
      the induction of germ cell apoptosis through the TNF-alpha/TNFR1 system and in 
      the modulation of the inflammatory process.
FAU - Rodriguez, Marcelo G
AU  - Rodriguez MG
AD  - Center for Research in Reproduction, School of Medicine, University of Buenos 
      Aires, Buenos Aires C1121 ABG, Argentina.
FAU - Rival, Claudia
AU  - Rival C
FAU - Theas, Maria S
AU  - Theas MS
FAU - Lustig, Livia
AU  - Lustig L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Disease Models, Animal
MH  - Functional Laterality
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/pathology
MH  - Spermatic Cord Torsion/*pathology/*surgery
MH  - Testis/*pathology
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2006/07/19 09:00
MHDA- 2006/10/27 09:00
CRDT- 2006/07/19 09:00
PHST- 2006/07/19 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2006/07/19 09:00 [entrez]
AID - 10.1111/j.1745-7262.2006.00146.x [doi]
PST - ppublish
SO  - Asian J Androl. 2006 Sep;8(5):576-83. doi: 10.1111/j.1745-7262.2006.00146.x.