PMID- 16848764
OWN - NLM
STAT- MEDLINE
DCOM- 20061128
LR  - 20181201
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 400
IP  - 2
DP  - 2006 Dec 1
TI  - EGFR-mediated expression of aquaporin-3 is involved in human skin fibroblast 
      migration.
PG  - 225-34
AB  - AQP3 (aquaporin-3), known as an integral membrane channel in epidermal 
      keratinocytes, facilitates water and glycerol movement into and out of the skin. 
      Here, we demonstrate that AQP3 is also expressed in cultured human skin 
      fibroblasts, which under normal wound healing processes migrate from surrounding 
      tissues to close the wound. EGF (epidermal growth factor), which induced 
      fibroblast migration, also induced AQP3 expression in a time- and dose-dependent 
      manner. CuSO4 and NiCl2, previously known as AQP3 water transport inhibitors, as 
      well as two other bivalent heavy metals Mn2+ and Co2+, inhibited EGF-induced cell 
      migration in human skin fibroblasts. AQP3 knockdown by small interfering RNA 
      inhibited EGF-induced AQP3 expression and cell migration. Furthermore, an EGFR 
      (EGF receptor) kinase inhibitor, PD153035, blocked EGF-induced AQP3 expression 
      and cell migration. MEK [MAPK (mitogen-activated protein kinase)/ERK 
      (extracellular-signal-regulated kinase) kinase]/ERK inhibitor U0126 and PI3K 
      (phosphoinositide 3-kinase) inhibitor LY294002 also inhibited EGF-induced AQP3 
      expression and cell migration. Collectively, our findings show for the first time 
      that AQP3 is expressed in human skin fibroblasts and that EGF induces AQP3 
      expression via EGFR, PI3K and ERK signal transduction pathways. We have provided 
      evidence for a novel role of AQP3 in human skin fibroblast cell migration, which 
      occurs during normal wound healing.
FAU - Cao, Cong
AU  - Cao C
AD  - Department of Biology, Providence College, 549 River Ave., Providence, RI 02918, 
      USA.
FAU - Sun, Yun
AU  - Sun Y
FAU - Healey, Sarah
AU  - Healey S
FAU - Bi, Zhigang
AU  - Bi Z
FAU - Hu, Gang
AU  - Hu G
FAU - Wan, Shu
AU  - Wan S
FAU - Kouttab, Nicola
AU  - Kouttab N
FAU - Chu, Wenming
AU  - Chu W
FAU - Wan, Yinsheng
AU  - Wan Y
LA  - eng
GR  - P20 RR016457/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (AQP3 protein, human)
RN  - 0 (Sulfates)
RN  - 158801-98-0 (Aquaporin 3)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 696BNE976J (nickel chloride)
RN  - 7OV03QG267 (Nickel)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
ECI - Biochem J. 2017 Aug 10;474(16):2901-2. PMID: 28798161
MH  - Aquaporin 3/*biosynthesis/genetics
MH  - Cell Movement/drug effects/*physiology
MH  - Cells, Cultured
MH  - Drug Interactions
MH  - Epidermal Growth Factor/antagonists & inhibitors/pharmacology
MH  - ErbB Receptors/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Fibroblasts/cytology/drug effects/enzymology/metabolism
MH  - Humans
MH  - Keratinocytes/cytology/drug effects/enzymology/metabolism
MH  - Nickel/pharmacology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - RNA Interference
MH  - Signal Transduction/drug effects
MH  - Skin/*cytology/drug effects/enzymology/*metabolism
MH  - Sulfates/pharmacology
MH  - Wound Healing/drug effects/physiology
PMC - PMC1652825
EDAT- 2006/07/20 09:00
MHDA- 2006/12/09 09:00
PMCR- 2007/06/01
CRDT- 2006/07/20 09:00
PHST- 2006/07/20 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/07/20 09:00 [entrez]
PHST- 2007/06/01 00:00 [pmc-release]
AID - BJ20060816 [pii]
AID - bj4000225 [pii]
AID - 10.1042/BJ20060816 [doi]
PST - ppublish
SO  - Biochem J. 2006 Dec 1;400(2):225-34. doi: 10.1042/BJ20060816.