PMID- 16849464
OWN - NLM
STAT- MEDLINE
DCOM- 20060906
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 177
IP  - 3
DP  - 2006 Aug 1
TI  - A critical role for prostaglandin E2 in podosome dissolution and induction of 
      high-speed migration during dendritic cell maturation.
PG  - 1567-74
AB  - Dendritic cells (DCs) are professional APCs of the immune system that play a key 
      role in regulating T cell-based immunity. The capacity of DCs to activate T cells 
      depends on their maturation state as well as their ability to migrate to the T 
      cell areas of draining lymph nodes. In this study, we investigated the effects of 
      DC maturation stimuli on the actin cytoskeleton and beta(1) integrin-dependent 
      adhesion and migration. Podosomes, specialized adhesion structures found in 
      immature monocyte-derived DCs as well as myeloid DCs, rapidly dissolve in 
      response to maturation stimuli such as TNF-alpha and PGE(2), whereas the TLR 
      agonist LPS induces podosome dissolution only after a long lag time. We 
      demonstrate that LPS-mediated podosome disassembly as well as the onset of 
      high-speed DC migration are dependent on the production of PGs by the DCs. 
      Moreover, both of these processes are inhibited by Ab-induced activation of 
      beta(1) integrins. Together, these results show that maturation-induced podosome 
      dissolution and loss of alpha(5)beta(1) integrin activity allow human DCs to 
      undergo the transition from an adhesive to a highly migratory phenotype.
FAU - van Helden, Suzanne F G
AU  - van Helden SF
AD  - Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, 
      Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands;
FAU - Krooshoop, Danielle J E B
AU  - Krooshoop DJ
FAU - Broers, Karin C M
AU  - Broers KC
FAU - Raymakers, Reinier A P
AU  - Raymakers RA
FAU - Figdor, Carl G
AU  - Figdor CG
FAU - van Leeuwen, Frank N
AU  - van Leeuwen FN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Integrin alpha5beta1)
RN  - 0 (Lipopolysaccharides)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Cell Adhesion/immunology
MH  - *Cell Differentiation/immunology
MH  - *Cell Movement/immunology
MH  - Cell Surface Extensions/immunology/*metabolism
MH  - Dendritic Cells/*cytology/*metabolism
MH  - Dinoprostone/biosynthesis/*physiology
MH  - Humans
MH  - Integrin alpha5beta1/antagonists & inhibitors/metabolism/physiology
MH  - Lipopolysaccharides/pharmacology
MH  - Myeloid Progenitor Cells/cytology/metabolism
MH  - Signal Transduction/immunology
MH  - Time Factors
EDAT- 2006/07/20 09:00
MHDA- 2006/09/07 09:00
CRDT- 2006/07/20 09:00
PHST- 2006/07/20 09:00 [pubmed]
PHST- 2006/09/07 09:00 [medline]
PHST- 2006/07/20 09:00 [entrez]
AID - 177/3/1567 [pii]
AID - 10.4049/jimmunol.177.3.1567 [doi]
PST - ppublish
SO  - J Immunol. 2006 Aug 1;177(3):1567-74. doi: 10.4049/jimmunol.177.3.1567.