PMID- 16849841
OWN - NLM
STAT- MEDLINE
DCOM- 20061003
LR  - 20190523
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 23
IP  - 6
DP  - 2006 Jun
TI  - Developmental profile of annexin IX and its possible role in programmed cell 
      death of the Bombyx mori anterior silk gland.
PG  - 533-42
AB  - During pupal metamorphosis, the anterior silk gland (ASG) of the silkworm, Bombyx 
      mori, undergoes programmed cell death (PCD), which is triggered by 
      20-hydroxyecdysone (20E). Annexin IX (ANX IX) has been identified as a 
      20E-inducible gene in dying ASGs, and we show here that its expression is 
      down-regulated in tissues destined to die but not in tissues that survive pupal 
      metamorphosis. ANX IX expression was high in the ASGs during the feeding period, 
      when the ecdysteroid titer was low, and decreased in response to the rising 
      ecdysteroid titer that triggered pupal metamorphosis. Before gut purge, in vitro 
      exposure of the ASGs to 20E levels corresponding to the ecdysteroid concentration 
      present at the time of gut purge caused a decrease in ANX IX messenger RNA 
      levels. Expression profiles of EcR and USP, and the 20E concentration-responses 
      of these genes, indicate the importance of the relative abundance of EcR-A and 
      EcR-B1 isoforms in ANX IX regulation. These results suggest an involvement of ANX 
      IX in the determination of PCD timing by delaying or suppressing the response to 
      the increase in hemolymph ecdysteroid concentration during the prepupal period.
FAU - Kaneko, Yu
AU  - Kaneko Y
AD  - Division of Life Sciences, Graduate School of Natural Science and Technology, 
      Kanazawa University, Japan.
FAU - Takaki, Keiko
AU  - Takaki K
FAU - Iwami, Masafumi
AU  - Iwami M
FAU - Sakurai, Sho
AU  - Sakurai S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
RN  - 0 (Annexins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (USP protein, Drosophila)
RN  - 5289-74-7 (Ecdysterone)
SB  - IM
MH  - Animals
MH  - Annexins/biosynthesis/*physiology
MH  - Apoptosis/*physiology
MH  - Bombyx/*physiology
MH  - DNA Primers/chemistry
MH  - DNA-Binding Proteins/physiology
MH  - Drosophila Proteins
MH  - Ecdysterone/physiology
MH  - Polymerase Chain Reaction/methods
MH  - Time Factors
MH  - Transcription Factors/physiology
EDAT- 2006/07/20 09:00
MHDA- 2006/10/04 09:00
CRDT- 2006/07/20 09:00
PHST- 2006/07/20 09:00 [pubmed]
PHST- 2006/10/04 09:00 [medline]
PHST- 2006/07/20 09:00 [entrez]
AID - JST.JSTAGE/zsj/23.533 [pii]
AID - 10.2108/zsj.23.533 [doi]
PST - ppublish
SO  - Zoolog Sci. 2006 Jun;23(6):533-42. doi: 10.2108/zsj.23.533.