PMID- 16850843
OWN - NLM
STAT- MEDLINE
DCOM- 20070126
LR  - 20161018
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 27
IP  - 5
DP  - 2006 May
TI  - [Detection of SRPs in injection water of Shenli Oil Field by FISH].
PG  - 972-6
AB  - Fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide 
      probes was applied for analyzing the structure of sulfate reducing prokaryotes 
      (SRPs) community in injection water of Shengli Oil Field. Eight probes and their 
      various combinations were used to detect SRPs in the water. Results showed SRPs 
      detected in the water were diverse, which followed in 4 bacterial phyla and 1 
      archaeal phylum. Total amount of SRPs was 2.86 x 10(4) cells/mL, accounting for 
      20% of total cells of microorganisms in the water of the Oil Field. Desulfovibrio 
      and Desulfotomaculum cells were about 8.71% (+/- 4.45%) and 12.15% (+/- 3.90%) of 
      the total microbial cells respectively, being dominant in the water. The relative 
      amounts of SRPs belonging to Desulfobacterales and Syntrophobacterales, 
      Thermodesulfobacteriales, and Thermodesulfovibro to total microbial cells in the 
      water were 7.59% (+/- 2.92%), 3.57% (+/- 1.39%) and 2.32% (+/- 0.80%) 
      respectively. In addition, SRPs belonging to Archaeoglobus were also detected 
      with the amount of 4.29% (+/- 1.75%) of total microbial cells, which tells that 
      archeal SRPs are also very important sulfate reducing microorganisms in the 
      injection water of oil field.
FAU - Zeng, Jing-hai
AU  - Zeng JH
AD  - ESPC State Key Joint Laboratory, Department of Environmental Science and 
      Engineering, Tsinghua University, Beijing 100084, China. 
      zengjh04@mails.gscas.ac.cn
FAU - Wu, Xiao-lei
AU  - Wu XL
FAU - Zhao, Gui-fang
AU  - Zhao GF
FAU - Qian, Yi
AU  - Qian Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
RN  - 0 (Petroleum)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfates)
SB  - IM
MH  - Archaea/growth & development/isolation & purification
MH  - Desulfitobacterium/growth & development/isolation & purification
MH  - Desulfotomaculum/growth & development/isolation & purification
MH  - Fresh Water/chemistry/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Petroleum
MH  - RNA, Bacterial/*analysis
MH  - RNA, Ribosomal, 16S/analysis
MH  - Sulfates/*metabolism
MH  - Sulfur-Reducing Bacteria/growth & development/*isolation & purification
MH  - *Water Microbiology
EDAT- 2006/07/21 09:00
MHDA- 2007/01/27 09:00
CRDT- 2006/07/21 09:00
PHST- 2006/07/21 09:00 [pubmed]
PHST- 2007/01/27 09:00 [medline]
PHST- 2006/07/21 09:00 [entrez]
PST - ppublish
SO  - Huan Jing Ke Xue. 2006 May;27(5):972-6.